Magnetic Compression of Tumor Spheroids Increases Cell Proliferation In Vitro and Cancer Progression In Vivo

A growing tumor is submitted to ever-evolving mechanical stress. Endoscopic procedures add additional constraints. However, the impact of mechanical forces on cancer progression is still debated. Herein, a set of magnetic methods is proposed to form tumor spheroids and to subject them to remote defo...

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Bibliographic Details
Published in:Cancers 2022-01, Vol.14 (2), p.366
Main Authors: Mary, Gaëtan, Malgras, Brice, Perez, Jose Efrain, Nagle, Irène, Luciani, Nathalie, Pimpie, Cynthia, Asnacios, Atef, Pocard, Marc, Reffay, Myriam, Wilhelm, Claire
Format: Article
Language:English
Subjects:
Adhesives
Cancer
Cell growth
Cell proliferation
Colon cancer
Colorectal cancer
Compression
Deformation
Glioblastoma
Glioblastoma cells
Hepatocytes
Hypoxia
Implants
Life Sciences
Malignancy
Matrix metalloproteinase
Metalloproteinase
Metastases
Mimicry
Nanoparticles
Peritoneum
Spheroids
Tumors
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Summary:A growing tumor is submitted to ever-evolving mechanical stress. Endoscopic procedures add additional constraints. However, the impact of mechanical forces on cancer progression is still debated. Herein, a set of magnetic methods is proposed to form tumor spheroids and to subject them to remote deformation, mimicking stent-imposed compression. Upon application of a permanent magnet, the magnetic tumor spheroids (formed from colon cancer cells or from glioblastoma cells) are compressed by 50% of their initial diameters. Such significant deformation triggers an increase in the spheroid proliferation for both cell lines, correlated with an increase in the number of proliferating cells toward its center and associated with an overexpression of the matrix metalloproteinase-9 (MMP-9). In vivo peritoneal injection of the spheroids made from colon cancer cells confirmed the increased aggressiveness of the compressed spheroids, with almost a doubling of the peritoneal cancer index (PCI), as compared with non-stimulated spheroids. Moreover, liver metastasis of labeled cells was observed only in animals grafted with stimulated spheroids. Altogether, these results demonstrate that a large compression of tumor spheroids enhances cancer proliferation and metastatic process and could have implications in clinical procedures where tumor compression plays a role.
ISSN:2072-6694
2072-6694
DOI:10.3390/cancers14020366