Enhanced Production of Bacterial Cellulose from Miscanthus as Sustainable Feedstock through Statistical Optimization of Culture Conditions

• Published in: International journal of environmental research and public health 2022-01, Vol.19 (2), p.866
• Main Authors: Son, Jemin, Lee, Kang Hyun, Lee, Taek, Kim, Hyun Soo, Shin, Weon Ho, Oh, Jong-Min, Koo, Sang-Mo, Yu, Byung Jo, Yoo, Hah Young, Park, Chulhwan
• Format: Article
• Language: English
• Subjects: Alginic acid; American dollar; Biomass; Biomaterials; Biomedical materials; Biorefineries; Carbon; Caustic soda; Cell culture; Cellulose; Climate change; Cosmetics; Culture Media; Deforestation; Design; Emissions; Emissions control; Experiments; Food; Food industry; Gluconacetobacter xylinus; Glucose; Glycerol; Greenhouse effect; Greenhouse gases; Hydrolysates; Lignocellulose; Miscanthus; Optimization; Petroleum industry; Potassium; Prediction models; Production costs; Raw materials; Sodium alginate; Statistical analysis; Statistics; Sustainability; Sustainable development; Variables; Variance analysis
• ISSN: 1660-4601; 1661-7827; 1660-4601
• DOI: 10.3390/ijerph19020866

Biorefineries are attracting attention as an alternative to the petroleum industry to reduce carbon emissions and achieve sustainable development. In particular, because forests play an important role in potentially reducing greenhouse gas emissions to net zero, alternatives to cellulose produced by plants are required. Bacterial cellulose (BC) can prevent deforestation and has a high potential for use as a biomaterial in various industries such as food, cosmetics, and pharmaceuticals. This study aimed to improve BC production from lignocellulose, a sustainable feedstock, and to optimize the culture conditions for using hydrolysates as a medium. The productivity of BC was improved using statistical optimization of the major culture parameters which were as follows: temperature, 29 °C; initial pH, 5.1; and sodium alginate concentration, 0.09% ( / ). The predicted and actual values of BC production in the optimal conditions were 14.07 g/L and 14.88 g/L, respectively, confirming that our prediction model was statistically significant. Additionally, BC production using hydrolysates was 1.12-fold higher than in the control group (commercial glucose). Our result indicate that lignocellulose can be used in the BC production processes in the near future.