Potential application of simple easy-to-use insertion-deletion (InDel) markers in citrus cultivar identification

We previously developed insertion-deletion (InDel) markers that distinguish three genotypes (two homozygous and one heterozygous) of diverse citrus cultivars. These InDel markers were codominant and could be clearly detected by using simple agarose gel electrophoresis. We sought to establish a metho...

Full description

Saved in:
Bibliographic Details
Published in:Breeding Science 2021, Vol.71(5), pp.601-608
Main Authors: Noda, Takahiro, Daiou, Kaoru, Mihara, Takashi, Nagano, Yukio
Format: Article
Language:English
Subjects:
Citrinae
Citrus
Citrus fruits
cultivar identification
Cultivars
Deletion
Electrophoresis
Fruits
Gel electrophoresis
genotype
Genotypes
Identification methods
InDel marker
Insertion
Markers
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We previously developed insertion-deletion (InDel) markers that distinguish three genotypes (two homozygous and one heterozygous) of diverse citrus cultivars. These InDel markers were codominant and could be clearly detected by using simple agarose gel electrophoresis. We sought to establish a method for cultivar identification using these 28 InDel markers to genotype 31 citrus cultivars. The results revealed that a minimum of 6 markers were required to identify individuals using the three-genotype classification method. Furthermore, we found that a simple method for distinguishing between two genotypes (homozygous and heterozygous) could be used to identify individuals using a minimum of 7 markers. Our findings provide a basis for the development of simple and rapid citrus cultivar identification methods.
ISSN:1344-7610
1347-3735
DOI:10.1270/jsbbs.21021