HI-NESS: a family of genetically encoded DNA labels based on a bacterial nucleoid-associated protein

Abstract The interplay between three-dimensional chromosome organisation and genomic processes such as replication and transcription necessitates in vivo studies of chromosome dynamics. Fluorescent organic dyes are often used for chromosome labelling in vivo. The mode of binding of these dyes to DNA...

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Published in:Nucleic acids research 2022-01, Vol.50 (2), p.e10-e10
Main Authors: Rashid, Fatema-Zahra M, Mahlandt, Eike, van der Vaart, Michiel, Boer, Daphne E C, Varela Alvarez, Monica, Henneman, Bram, Brocken, Daan J W, Voskamp, Patrick, Blok, Anneloes J, Shimizu, Thomas S, Meijer, Annemarie H, Luijsterburg, Martijn S, Goedhart, Joachim, Crémazy, Frédéric G E, Dame, Remus T
Format: Article
Language:English
Subjects:
Animals
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biological Assay - methods
Cell Line
Cloning, Molecular
DNA Replication
DNA, Bacterial - chemistry
DNA, Bacterial - metabolism
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Fluorescent Dyes
Gene Expression
Genetic Vectors
Life Sciences
Methods Online
Microscopy, Fluorescence
Staining and Labeling - methods
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Summary:Abstract The interplay between three-dimensional chromosome organisation and genomic processes such as replication and transcription necessitates in vivo studies of chromosome dynamics. Fluorescent organic dyes are often used for chromosome labelling in vivo. The mode of binding of these dyes to DNA cause its distortion, elongation, and partial unwinding. The structural changes induce DNA damage and interfere with the binding dynamics of chromatin-associated proteins, consequently perturbing gene expression, genome replication, and cell cycle progression. We have developed a minimally-perturbing, genetically encoded fluorescent DNA label consisting of a (photo-switchable) fluorescent protein fused to the DNA-binding domain of H-NS — a bacterial nucleoid-associated protein. We show that this DNA label, abbreviated as HI-NESS (H-NS-based indicator for nucleic acid stainings), is minimally-perturbing to genomic processes and labels chromosomes in eukaryotic cells in culture, and in zebrafish embryos with preferential binding to AT-rich chromatin.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/gkab993