Obesity and Roux-en-Y gastric bypass drive changes in miR-31 and miR-215 expression in the human rectal mucosa

Background/Objectives Obesity increases colorectal cancer (CRC) risk. However, the effects of weight loss on CRC risk are unclear. Epigenetic mechanisms involving microRNAs that lead to dysregulated gene expression may mediate the effects of obesity and weight loss on CRC risk. We examined the effec...

Full description

Saved in:
Bibliographic Details
Published in:International Journal of Obesity 2022-02, Vol.46 (2), p.333-341
Main Authors: Breininger, Stella Panagio, Sabater, Laura, Malcomson, Fiona Caroline, Afshar, Sorena, Mann, Jelena, Mathers, John Cummings
Format: Article
Language:English
Subjects:
38/91
45/77
631/208
631/67
Adult
Bioinformatics
Biopsy
Body mass
Body weight loss
Cancer
Colorectal cancer
Colorectal carcinoma
Colorectal Neoplasms - epidemiology
Colorectal Neoplasms - etiology
Colorectal Neoplasms - metabolism
England - epidemiology
Epidemiology
Epigenetics
Female
Gastric bypass
Gastric Bypass - adverse effects
Gastric Bypass - methods
Gastric Bypass - statistics & numerical data
Gastrointestinal surgery
Gene expression
Health Promotion and Disease Prevention
Humans
Internal Medicine
Male
Medicine
Medicine & Public Health
Metabolic Diseases
MicroRNAs
MicroRNAs - analysis
Middle Aged
miRNA
Mucosa
Mucous Membrane - metabolism
Mucous Membrane - physiopathology
Next-generation sequencing
Obesity
Obesity - epidemiology
Obesity - metabolism
Obesity - physiopathology
Polymerase chain reaction
Public Health
Rectum
Rectum - metabolism
Rectum - physiopathology
Ribonucleic acid
Risk
RNA
Statistics, Nonparametric
Weight control
Weight loss
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background/Objectives Obesity increases colorectal cancer (CRC) risk. However, the effects of weight loss on CRC risk are unclear. Epigenetic mechanisms involving microRNAs that lead to dysregulated gene expression may mediate the effects of obesity and weight loss on CRC risk. We examined the effects of obesity and weight loss following Roux-en-Y gastric bypass (RYGB) on microRNA expression in the human rectal mucosa. Subjects/Methods We collected rectal mucosal biopsies from obese patients ( n  = 22) listed for RYGB and age- and sex-matched healthy non-obese Controls ( n  = 20), at baseline and six months post-surgery. We quantified microRNA expression in rectal mucosal biopsies using Next Generation Sequencing and bioinformatics analysis to investigate the likely functional consequences of these epigenetic changes. Results Compared with non-obese individuals, obese individuals showed differential expression of 112 microRNAs (p 
ISSN:0307-0565
1476-5497
DOI:10.1038/s41366-021-01005-y