Correlation between Legionella pneumophila serogroups isolated from patients with ventilator-associated pneumonia and water resources: a study of four hospitals in Tehran, Iran

• Published in: Environmental science and pollution research international 2022-06, Vol.29 (27), p.41368-41374
• Main Authors: Sakhaee, Fatemeh, Mafi, Shirin, Zargar, Mohsen, Vaziri, Farzam, Hajiesmaeili, Mohammadreza, Siadat, Seyed Davar, Fateh, Abolfazl
• Format: Article
• Language: English
• Subjects: Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Bronchus; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Environmental science; Gene sequencing; Genetic diversity; Hospitals; Humans; Infectivity; Iran; Lavage; Legionella; Legionella pneumophila; Legionnaires' Disease - epidemiology; Legionnaires' disease bacterium; Patients; Pneumonia; Pneumonia, Ventilator-Associated - epidemiology; Polymerase chain reaction; Real-Time Polymerase Chain Reaction; Research Article; Respiratory tract; Respiratory tract diseases; Serogroup; Ventilator-associated pneumonia; Ventilators; Waste Water Technology; Water; Water analysis; Water Management; Water Microbiology; Water Pollution Control; Water resources; Water sampling
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-022-18867-y

Legionella pneumophila ( L. pneumophila ) is one of the main pathogens, causing pneumonia and respiratory tract infections, especially in patients with ventilator-associated pneumonia (VAP). This study aimed to approve the hypothesis that the serogroup distribution of L. pneumophila isolates from patients is correlated with Legionella strains in the environment. A total of 280 bronchoalveolar lavage (BAL) samples from VAP patients admitted to the intensive care unit (ICU) as well as 116 water samples from different sources in four hospitals in Tehran, Iran, were evaluated for the presence of L. pneumophila infection by culture, nested polymerase chain reaction (PCR), real-time PCR, and sequencing for genetic diversity. The molecular and culture methods found 24 (8.6%) and 5 (1.8%) samples to be positive for L. pneumophila in VAP patients, while they found 23 (19.8%) and 8 (6.9%) positive samples in water resources, respectively. The sequencing results indicated that all positive clinical samples and 14 (60.8%) environmental samples were belonged to L. pneumophila serogroup 1. Smoking, age, length of ICU stay, and duration of ventilator use had strong relationship with L. pneumophila infectivity. In conclusion, this is the first report from Iran to determine minor differences in the serogroup distribution of environmental and clinical strains. However, further studies are needed to confirm this relationship in different regions of Iran.