Long-Term Potentiation of Mossy Fiber Feedforward Inhibition of CA3 Pyramidal Cells Maintains E/I Balance in Epilepsy Model

• Published in: eNeuro 2022-01, Vol.9 (1), p.ENEURO.0375-21.2021
• Main Authors: Pan, Enhui, Puranam, Ram S, McNamara, James O
• Format: Article
• Language: English
• Subjects: Animals; Epilepsy; Epilepsy, Temporal Lobe; Long-Term Potentiation; Mice; Mossy Fibers, Hippocampal; New Research; Pyramidal Cells
• ISSN: 2373-2822; 2373-2822
• DOI: 10.1523/ENEURO.0375-21.2021

Insight into the cellular and circuit mechanisms underlying development of temporal lobe epilepsy (TLE) will provide a foundation for improved therapies. We studied a model in which an episode of prolonged seizures is followed by recovery lasting two weeks before emergence of spontaneous recurrent seizures. We focused on the interval between the prolonged seizures and the late onset recurrent seizures. We investigated the hippocampal mossy fiber CA3 pyramidal cell microcircuit in models spanning , , and preparations. Expression of channelrhodopsin-2 in the dentate granule cells of mice enabled the selective activation of mossy fiber axons. studies revealed marked potentiation of mossy fiber evoked field potentials in hippocampal CA3 beginning within hours following seizures, a potentiation which persisted at least 7 d. Stimulation of mossy fibers in hippocampal slices using patterns of activity mimicking seizures induced LTP not only of the monosynaptic EPSC but also of the disynaptic IPSC of CA3 pyramidal cells. studies of slices isolated following seizures revealed evidence of LTP of mossy fiber evoked EPSC and disynaptic IPSC of CA3 pyramidal cells. We suggest that activation of dentate granule cells during seizures induces these plasticities and the retained balance of synaptic excitation and inhibition limits excessive activation of CA3 pyramidal cells, thereby protecting animals from spontaneous recurrent seizures at this interval following status epilepticus.