Long intergenic non-protein coding RNA 02570 promotes nasopharyngeal carcinoma progression by adsorbing microRNA miR-4649-3p thereby upregulating both sterol regulatory element binding protein 1, and fatty acid synthase

• Published in: Bioengineered 2021-01, Vol.12 (1), p.7108-7119
• Main Authors: Liu, Fei, Wei, Jiazhang, Hao, Yanrong, Lan, Jiao, Li, Wei, Weng, Jingjin, Li, Min, Su, Cheng, Li, Bing, Mo, Mingzheng, Tang, Fengzhu, Wang, Yongli, Yang, Yong, Jiao, Wei, Qu, Shenhong
• Format: Article
• Language: English
• Subjects: Cell Line, Tumor; Disease Progression; FASN; Fatty Acid Synthase, Type I - genetics; Fatty Acid Synthase, Type I - metabolism; Humans; LINC02570; MicroRNAs - genetics; MicroRNAs - metabolism; miR-4649-3p; nasopharyngeal carcinoma (NPC); Nasopharyngeal Carcinoma - genetics; Nasopharyngeal Carcinoma - metabolism; Nasopharyngeal Carcinoma - mortality; Nasopharyngeal Carcinoma - pathology; Nasopharyngeal Neoplasms - genetics; Nasopharyngeal Neoplasms - metabolism; Nasopharyngeal Neoplasms - mortality; Nasopharyngeal Neoplasms - pathology; Nasopharynx - pathology; progression; Research Paper; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; SREBP1; Sterol Regulatory Element Binding Protein 1 - genetics; Sterol Regulatory Element Binding Protein 1 - metabolism; Up-Regulation
• ISSN: 2165-5979; 2165-5987
• DOI: 10.1080/21655979.2021.1979317

Our previous studies have elucidated a possible connection between long intergenic non-protein coding RNA 2570 (LINC02570) and nasopharyngeal carcinoma (NPC). However, the precise mechanism by which LINC02570 promotes NPC remains unknown. We used quantitative polymerase chain reaction (qPCR) to detect LINC02570 expression in nasopharyngeal cell lines, NPC tissues, and chronic rhinitis tissues. Subcellular LINC02570 localization was confirmed by fluorescence in situ hybridization (FISH). The effects of LINC02570 stable knockdown and overexpression on viabillity, proliferation, migration, and invasion were analyzed using 3-(4,5-Dimethyl-2-Thiazolyl)-2,5-Diphenyl-2-H-Tetrazolium bromide (MTT), a colorimetric focus-formation assay, a wound healing assay, and transwell assays. RNA crosstalk analysis in silico predicted microRNA-4649-3p (miR-4649-3p) binding to LINC02570 or sterol regulatory element binding transcription factor 1 (SREBF1). A dual luciferase reporter assay was used to confirm potential interactions. Sterol regulatory element binding protein 1 (SREBP1) and fatty acid synthase (FASN) expression were detected by western blotting. The results suggest that LINC02570 is upregulated in late clinical stage NPC patients, and promotes NPC progression by adsorbing miR-4649-3p to up-regulate SREBP1 and FASN. This study elucidates a potential chemotherapeutic target involved in lipid metabolism in NPC.