Molecular Mechanism of Biofilm Locator Protein Kinase Dbf2p-related kinase 1 in Regulating Innate Immune Response to Interleukin 17-induced Viral Pneumonia

It focused on the antiviral immune regulation of biofilm-localized protein kinase Dbf2p-related kinase 1 (NDR1) in viral pneumonia. Mouse alveolar monocyte RAW264.7 was used as blank control, and viral pneumonia cell model was prepared by infecting cells with respiratory syncytial virus (RSV). NDR1...

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Bibliographic Details
Published in:Bioengineered 2021-12, Vol.12 (2), p.10335-10344
Main Authors: Wang, Haifeng, Bu, Lina, Shu, Fang, Bai, Yun, Xue, Feixiao, Shi, Shanshan, Sun, Daqing
Format: Article
Language:English
Subjects:
Animals
Biofilms - growth & development
Cell Proliferation
Chemokines - metabolism
IL17
immune response
Immunity, Innate
Inflammation - pathology
inflammatory responses
Interleukin-17 - metabolism
Mice
NDR1
Pneumonia, Viral - enzymology
Pneumonia, Viral - immunology
Protein Serine-Threonine Kinases
RAW 264.7 Cells
RAW264.7
Research Paper
respiratory syncytial virus
Respiratory Syncytial Virus Infections - immunology
Respiratory Syncytial Virus Infections - virology
Respiratory Syncytial Viruses - physiology
Signal Transduction
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Summary:It focused on the antiviral immune regulation of biofilm-localized protein kinase Dbf2p-related kinase 1 (NDR1) in viral pneumonia. Mouse alveolar monocyte RAW264.7 was used as blank control, and viral pneumonia cell model was prepared by infecting cells with respiratory syncytial virus (RSV). NDR1 overexpression vector and siRNA interference sequences were synthesized, and overexpression/silence NDR1 cell model was fabricated. About 50 ng/mL interleukin 17 (IL-17) was given to stimulate. Enzyme-linked immunosorbent assay (ELISA), quantitative reverse transcription PCR (RT-qRCR), and Western blot were performed to detect cytokines and chemokines, mRNA of inflammatory factors, and signal molecule protein expression. Notably, RSV infection increased RSV-F mRNA in RAW264.7 cells and reduced NDR1 mRNA and protein. Secretion levels of IL-6, interferon β (IFN-β), chemokine (C-X-C motif) ligand 2 (CXCL2), and chemokine (C-C motif) ligand 2 (CCL20) increased in the model group versus blank control (P
ISSN:2165-5979
2165-5987
DOI:10.1080/21655979.2021.1996316