Genotyping Encephalitozoon hellem isolates by analysis of the polar tube protein gene

To develop an alternative genotyping tool, the genetic diversity of Encephalitozoon hellem was examined at the polar tube protein (PTP) locus. Nucleotide sequence analysis of the PTP gene divided 24 E. hellem isolates into four genotypes, compared to two genotypes identified by analysis of the inter...

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Bibliographic Details
Published in:Journal of clinical microbiology 2001-06, Vol.39 (6), p.2191-2196
Main Authors: LIHUA XIAO, LIXIA LI, MOURA, Hercules, SULAIMAN, Irshad, LAL, Altaf A, GATTI, Simonetta, SCAGLIA, Massimo, DIDIER, Elizabeth S, VISVESVARA, Govinda S
Format: Article
Language:English
Subjects:
Animals
Bacteriology
Base Sequence
Biological and medical sciences
DNA, Ribosomal Spacer - genetics
Encephalitozoon - classification
Encephalitozoon - genetics
Encephalitozoonosis - parasitology
Epidemiology
Fundamental and applied biological sciences. Psychology
Fungal Proteins
Genes, Protozoan
Genetics
Genotype
Humans
Microbiology
Molecular Sequence Data
Parasitology
Polymerase Chain Reaction
Protozoan Proteins - genetics
RNA, Ribosomal - genetics
Sequence Analysis, DNA
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Summary:To develop an alternative genotyping tool, the genetic diversity of Encephalitozoon hellem was examined at the polar tube protein (PTP) locus. Nucleotide sequence analysis of the PTP gene divided 24 E. hellem isolates into four genotypes, compared to two genotypes identified by analysis of the internal transcribed spacer of the rRNA gene. The four PTP genotypes differed from each other by the copy number of the 60-bp central repeat as well as by point mutations. A simple PCR test was developed to differentiate E. hellem genotypes based on the difference in the size of PTP PCR products, which should facilitate the genotyping of E. hellem in clinical samples.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.39.6.2191-2196.2001