Expression of inflammatory mediators in biofilm samples and clinical association in inflammatory bowel disease patients—a preliminary study

• Published in: Clinical oral investigations 2022-02, Vol.26 (2), p.1217-1228
• Main Authors: Buchbender, Mayte, Fehlhofer, Jakob, Proff, Peter, Möst, Tobias, Ries, Jutta, Hannig, Matthias, Neurath, Markus F., Gund, Madline, Atreya, Raja, Kesting, Marco
• Format: Article
• Language: English
• Subjects: Antigens; Biofilms; Cadmium; CD11a antigen; Clinical trials; Colitis, Ulcerative; Crohn Disease; Crohn's disease; Cytokines; Dentistry; Gene expression; Gum disease; Humans; Immunosuppressive agents; Inflammation Mediators; Inflammatory bowel disease; Inflammatory Bowel Diseases; Interleukin 10; Interleukin 2; Intestine; Matrilysin; Matrix metalloproteinase; Medicine; Oral cavity; Original; Original Article; Patients; Plaque index; Surface antigens; Tumor necrosis factor; Ulcerative colitis
• ISSN: 1432-6981; 1436-3771
• DOI: 10.1007/s00784-021-04093-2

Objectives Inflammatory bowel disease (IBD) has multiple impacts on soft and hard tissues in the oral cavity. The aim of this study was to analyze the expression of cytokines in biofilm samples from patients suffering from IBD and compare them to healthy patients. It was hypothesized that different cytokine expression levels and clinical associations might be drawn. Material and methods A total of 56 biofilm samples from three different patient cohorts (group 0 = healthy, HC n  = 30; group 1 = Crohn’s disease, CD, n  = 19; group 2 = ulcerative colitis, UC, n  = 7) were examined for the expression levels of the cytokine interleukins IL-2, -6, and -10; matrix metalloproteinases 7 and 9; and surface antigens CD90/CD11a by quantitative real-time PCR and according to clinical parameters (plaque index, BOP, PD, DMFT, CAL). Relative gene expression was determined using the ∆∆CT method. Results The mean BOP values ( p  = 0.001) and PD ( p  = 0.000) were significantly higher in the CD group compared to controls. Expression of IL-10 was significantly higher in the CD ( p  = 0.004) and UC groups ( p  = 0.022). Expression of MMP-7 was significantly higher in the CD group ( p  = 0.032). IBD patients treated with TNF inhibitors ( p  = 0.007) or other immunosuppressants ( p  = 0.014) showed significant overexpression of IL-10 compared to controls. Conclusion Different expression levels of IL-10 and MMP-7 were detected in plaque samples from IBD patients. As only BOP was significantly increased, we conclude that no clinical impairment of periodontal tissue occurred in IBD patients. Clinical relevance With the worldwide increasing incidence of IBD, it is important to obtain insights into the effects of the disease on the oral cavity. The study was registered (01.09.2020) at the German clinical trial registry (DRKS00022956). Clinical trial registration The study is registered at the German clinical trial registry (DRKS00022956).