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PDRG1 promotes the proliferation and migration of GBM cells by the MEK/ERK/CD44 pathway

P53 and DNA damage‐regulated gene1 (PDRG1) is overexpressed in diverse carcinomas. Here, we discover that PDRG1 is overexpressed in glioblastoma multiforme (GBM). However, the clinical significance, biological role, and underlying molecular mechanisms of PDRG1 in GBM remain unclear. PDRG1 was aberra...

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Published in:Cancer science 2022-02, Vol.113 (2), p.500-516
Main Authors: Sun, Jinmin, Xu, Yixin, Liu, Jia, Cui, Huiyue, Cao, Haowei, Ren, Jing
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Liu, Jia
Cui, Huiyue
Cao, Haowei
Ren, Jing
description P53 and DNA damage‐regulated gene1 (PDRG1) is overexpressed in diverse carcinomas. Here, we discover that PDRG1 is overexpressed in glioblastoma multiforme (GBM). However, the clinical significance, biological role, and underlying molecular mechanisms of PDRG1 in GBM remain unclear. PDRG1 was aberrantly overexpressed in glioma, especially prevalent in GBM, and correlated with poor clinicopathologic features of glioma. The risk score, operational feature curve analysis, Kaplan‐Meier curve, and univariate and multivariate Cox regression analysis indicated that PDRG1 was an independent prognostic indicator and significantly correlates with disease progression of glioma. A prognostic nomogram was constructed to predict the survival risk of individual patients. The function and pathway enrichment analysis of PDRG1 in The Cancer Genome Atlas cohort was performed. PDRG1 knockdown significantly inhibited the migration and proliferation of GBM cells in vitro and in vivo. Transcriptome sequencing analysis of PDRG1 knockdown U‐118 MG(U118) cells indicated that biological regulation adhesion, growth and death, cell motility, cell adhesion molecular and proteoglycans in cancer were significantly enriched. Importantly, we found that the expression of adhesion molecule cluster of differentiation 44 (CD44) was regulated by PDRG1 in GBM. We found that PDRG1 promoted the migration and proliferation of GBM cells via the mitogen‐activated protein kinase kinase (MEK)/extracellular regulated protein kinase (ERK)/CD44 pathway. Our findings provide proof that PDRG1 upregulation predicts progression and poor prognosis in human gliomas, especially in isocitrate dehydrogenase (IDH) wt glioma patients. The study provides new evidence that PDRG1 regulates the expression of CD44 in GBM cells and might promote the migration and proliferation via the MEK/ERK/CD44pathway. PDRG1 might be a novel diagnostic indicator and promising therapeutic target for GBM. Our study provides the evidence that aberrantly expressed P53 and DNA damage‐regulated gene1 (PDRG1) might be a negative prognosticator in glioma. We characterized the roles of PDRG1 in proliferation and migration of GBM cells, and delineated the underlying molecular mechanism that PDRG1 functions via the MEK/extracellular regulated protein kinase/cluster of differentiation 44 pathway. Our data provide evidence that PDRG1 might serve as a valuable prognostic marker. Inhibition of PDRG1 may be a plausible strategy for the development of
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Here, we discover that PDRG1 is overexpressed in glioblastoma multiforme (GBM). However, the clinical significance, biological role, and underlying molecular mechanisms of PDRG1 in GBM remain unclear. PDRG1 was aberrantly overexpressed in glioma, especially prevalent in GBM, and correlated with poor clinicopathologic features of glioma. The risk score, operational feature curve analysis, Kaplan‐Meier curve, and univariate and multivariate Cox regression analysis indicated that PDRG1 was an independent prognostic indicator and significantly correlates with disease progression of glioma. A prognostic nomogram was constructed to predict the survival risk of individual patients. The function and pathway enrichment analysis of PDRG1 in The Cancer Genome Atlas cohort was performed. PDRG1 knockdown significantly inhibited the migration and proliferation of GBM cells in vitro and in vivo. Transcriptome sequencing analysis of PDRG1 knockdown U‐118 MG(U118) cells indicated that biological regulation adhesion, growth and death, cell motility, cell adhesion molecular and proteoglycans in cancer were significantly enriched. Importantly, we found that the expression of adhesion molecule cluster of differentiation 44 (CD44) was regulated by PDRG1 in GBM. We found that PDRG1 promoted the migration and proliferation of GBM cells via the mitogen‐activated protein kinase kinase (MEK)/extracellular regulated protein kinase (ERK)/CD44 pathway. Our findings provide proof that PDRG1 upregulation predicts progression and poor prognosis in human gliomas, especially in isocitrate dehydrogenase (IDH) wt glioma patients. The study provides new evidence that PDRG1 regulates the expression of CD44 in GBM cells and might promote the migration and proliferation via the MEK/ERK/CD44pathway. PDRG1 might be a novel diagnostic indicator and promising therapeutic target for GBM. Our study provides the evidence that aberrantly expressed P53 and DNA damage‐regulated gene1 (PDRG1) might be a negative prognosticator in glioma. We characterized the roles of PDRG1 in proliferation and migration of GBM cells, and delineated the underlying molecular mechanism that PDRG1 functions via the MEK/extracellular regulated protein kinase/cluster of differentiation 44 pathway. Our data provide evidence that PDRG1 might serve as a valuable prognostic marker. Inhibition of PDRG1 may be a plausible strategy for the development of the target therapy of glioma.</description><identifier>ISSN: 1347-9032</identifier><identifier>EISSN: 1349-7006</identifier><identifier>DOI: 10.1111/cas.15214</identifier><identifier>PMID: 34812552</identifier><language>eng</language><publisher>England: John Wiley &amp; Sons, Inc</publisher><subject>Animals ; Bioinformatics ; Brain cancer ; Cancer therapies ; Carcinoma ; CD44 antigen ; Cell adhesion ; Cell cycle ; Cell death ; Cell growth ; Cell Line, Tumor ; Cell migration ; Cell Movement ; Cell Proliferation ; Chemotherapy ; Committees ; Correlation analysis ; Datasets ; Disease Progression ; DNA damage ; DNA methylation ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; Extracellular regulated protein kinase ; Extracellular signal-regulated kinase ; Female ; Gene expression ; Gene Expression Regulation, Neoplastic ; Genomes ; Genotype &amp; phenotype ; Glioblastoma ; Glioblastoma - genetics ; Glioblastoma - metabolism ; Glioblastoma - pathology ; Glioblastoma multiforme ; Glioma ; Glioma - genetics ; Glioma - metabolism ; Glioma - pathology ; Humans ; Hyaluronan Receptors - metabolism ; Isocitrate dehydrogenase ; Kinases ; Laboratory animals ; Lung cancer ; Male ; MAP Kinase Signaling System ; Medical prognosis ; Mice ; Middle Aged ; migration ; Molecular modelling ; Original ; P53 and DNA damage‐regulated gene1 ; p53 Protein ; Patients ; Prognosis ; proliferation ; Protein kinase ; Proteins ; Proteoglycans ; Radiation ; Reagents ; Sequence analysis ; Signal Transduction ; Software ; Therapeutic targets ; Transcriptomes</subject><ispartof>Cancer science, 2022-02, Vol.113 (2), p.500-516</ispartof><rights>2021 The Authors. published by John Wiley &amp; Sons Australia, Ltd on behalf of Japanese Cancer Association.</rights><rights>2021 The Authors. Cancer Science published by John Wiley &amp; Sons Australia, Ltd on behalf of Japanese Cancer Association.</rights><rights>2022. This work is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). 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Here, we discover that PDRG1 is overexpressed in glioblastoma multiforme (GBM). However, the clinical significance, biological role, and underlying molecular mechanisms of PDRG1 in GBM remain unclear. PDRG1 was aberrantly overexpressed in glioma, especially prevalent in GBM, and correlated with poor clinicopathologic features of glioma. The risk score, operational feature curve analysis, Kaplan‐Meier curve, and univariate and multivariate Cox regression analysis indicated that PDRG1 was an independent prognostic indicator and significantly correlates with disease progression of glioma. A prognostic nomogram was constructed to predict the survival risk of individual patients. The function and pathway enrichment analysis of PDRG1 in The Cancer Genome Atlas cohort was performed. PDRG1 knockdown significantly inhibited the migration and proliferation of GBM cells in vitro and in vivo. Transcriptome sequencing analysis of PDRG1 knockdown U‐118 MG(U118) cells indicated that biological regulation adhesion, growth and death, cell motility, cell adhesion molecular and proteoglycans in cancer were significantly enriched. Importantly, we found that the expression of adhesion molecule cluster of differentiation 44 (CD44) was regulated by PDRG1 in GBM. We found that PDRG1 promoted the migration and proliferation of GBM cells via the mitogen‐activated protein kinase kinase (MEK)/extracellular regulated protein kinase (ERK)/CD44 pathway. Our findings provide proof that PDRG1 upregulation predicts progression and poor prognosis in human gliomas, especially in isocitrate dehydrogenase (IDH) wt glioma patients. The study provides new evidence that PDRG1 regulates the expression of CD44 in GBM cells and might promote the migration and proliferation via the MEK/ERK/CD44pathway. PDRG1 might be a novel diagnostic indicator and promising therapeutic target for GBM. 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phenotype</topic><topic>Glioblastoma</topic><topic>Glioblastoma - genetics</topic><topic>Glioblastoma - metabolism</topic><topic>Glioblastoma - pathology</topic><topic>Glioblastoma multiforme</topic><topic>Glioma</topic><topic>Glioma - genetics</topic><topic>Glioma - metabolism</topic><topic>Glioma - pathology</topic><topic>Humans</topic><topic>Hyaluronan Receptors - metabolism</topic><topic>Isocitrate dehydrogenase</topic><topic>Kinases</topic><topic>Laboratory animals</topic><topic>Lung cancer</topic><topic>Male</topic><topic>MAP Kinase Signaling System</topic><topic>Medical prognosis</topic><topic>Mice</topic><topic>Middle Aged</topic><topic>migration</topic><topic>Molecular modelling</topic><topic>Original</topic><topic>P53 and DNA damage‐regulated gene1</topic><topic>p53 Protein</topic><topic>Patients</topic><topic>Prognosis</topic><topic>proliferation</topic><topic>Protein kinase</topic><topic>Proteins</topic><topic>Proteoglycans</topic><topic>Radiation</topic><topic>Reagents</topic><topic>Sequence analysis</topic><topic>Signal Transduction</topic><topic>Software</topic><topic>Therapeutic targets</topic><topic>Transcriptomes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sun, Jinmin</creatorcontrib><creatorcontrib>Xu, Yixin</creatorcontrib><creatorcontrib>Liu, Jia</creatorcontrib><creatorcontrib>Cui, Huiyue</creatorcontrib><creatorcontrib>Cao, Haowei</creatorcontrib><creatorcontrib>Ren, Jing</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>Wiley Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Biological Science Collection</collection><collection>ProQuest Biological Science Journals</collection><collection>ProQuest - Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cancer science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sun, Jinmin</au><au>Xu, Yixin</au><au>Liu, Jia</au><au>Cui, Huiyue</au><au>Cao, Haowei</au><au>Ren, Jing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PDRG1 promotes the proliferation and migration of GBM cells by the MEK/ERK/CD44 pathway</atitle><jtitle>Cancer science</jtitle><addtitle>Cancer Sci</addtitle><date>2022-02</date><risdate>2022</risdate><volume>113</volume><issue>2</issue><spage>500</spage><epage>516</epage><pages>500-516</pages><issn>1347-9032</issn><eissn>1349-7006</eissn><abstract>P53 and DNA damage‐regulated gene1 (PDRG1) is overexpressed in diverse carcinomas. Here, we discover that PDRG1 is overexpressed in glioblastoma multiforme (GBM). However, the clinical significance, biological role, and underlying molecular mechanisms of PDRG1 in GBM remain unclear. PDRG1 was aberrantly overexpressed in glioma, especially prevalent in GBM, and correlated with poor clinicopathologic features of glioma. The risk score, operational feature curve analysis, Kaplan‐Meier curve, and univariate and multivariate Cox regression analysis indicated that PDRG1 was an independent prognostic indicator and significantly correlates with disease progression of glioma. A prognostic nomogram was constructed to predict the survival risk of individual patients. The function and pathway enrichment analysis of PDRG1 in The Cancer Genome Atlas cohort was performed. PDRG1 knockdown significantly inhibited the migration and proliferation of GBM cells in vitro and in vivo. Transcriptome sequencing analysis of PDRG1 knockdown U‐118 MG(U118) cells indicated that biological regulation adhesion, growth and death, cell motility, cell adhesion molecular and proteoglycans in cancer were significantly enriched. Importantly, we found that the expression of adhesion molecule cluster of differentiation 44 (CD44) was regulated by PDRG1 in GBM. We found that PDRG1 promoted the migration and proliferation of GBM cells via the mitogen‐activated protein kinase kinase (MEK)/extracellular regulated protein kinase (ERK)/CD44 pathway. Our findings provide proof that PDRG1 upregulation predicts progression and poor prognosis in human gliomas, especially in isocitrate dehydrogenase (IDH) wt glioma patients. The study provides new evidence that PDRG1 regulates the expression of CD44 in GBM cells and might promote the migration and proliferation via the MEK/ERK/CD44pathway. PDRG1 might be a novel diagnostic indicator and promising therapeutic target for GBM. Our study provides the evidence that aberrantly expressed P53 and DNA damage‐regulated gene1 (PDRG1) might be a negative prognosticator in glioma. We characterized the roles of PDRG1 in proliferation and migration of GBM cells, and delineated the underlying molecular mechanism that PDRG1 functions via the MEK/extracellular regulated protein kinase/cluster of differentiation 44 pathway. Our data provide evidence that PDRG1 might serve as a valuable prognostic marker. Inhibition of PDRG1 may be a plausible strategy for the development of the target therapy of glioma.</abstract><cop>England</cop><pub>John Wiley &amp; Sons, Inc</pub><pmid>34812552</pmid><doi>10.1111/cas.15214</doi><tpages>17</tpages><orcidid>https://orcid.org/0000-0003-1384-7369</orcidid><oa>free_for_read</oa></addata></record>
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ispartof Cancer science, 2022-02, Vol.113 (2), p.500-516
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1349-7006
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8819344
source PubMed (Medline); Wiley Online Library Open Access; ProQuest - Publicly Available Content Database
subjects Animals
Bioinformatics
Brain cancer
Cancer therapies
Carcinoma
CD44 antigen
Cell adhesion
Cell cycle
Cell death
Cell growth
Cell Line, Tumor
Cell migration
Cell Movement
Cell Proliferation
Chemotherapy
Committees
Correlation analysis
Datasets
Disease Progression
DNA damage
DNA methylation
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Extracellular regulated protein kinase
Extracellular signal-regulated kinase
Female
Gene expression
Gene Expression Regulation, Neoplastic
Genomes
Genotype & phenotype
Glioblastoma
Glioblastoma - genetics
Glioblastoma - metabolism
Glioblastoma - pathology
Glioblastoma multiforme
Glioma
Glioma - genetics
Glioma - metabolism
Glioma - pathology
Humans
Hyaluronan Receptors - metabolism
Isocitrate dehydrogenase
Kinases
Laboratory animals
Lung cancer
Male
MAP Kinase Signaling System
Medical prognosis
Mice
Middle Aged
migration
Molecular modelling
Original
P53 and DNA damage‐regulated gene1
p53 Protein
Patients
Prognosis
proliferation
Protein kinase
Proteins
Proteoglycans
Radiation
Reagents
Sequence analysis
Signal Transduction
Software
Therapeutic targets
Transcriptomes
title PDRG1 promotes the proliferation and migration of GBM cells by the MEK/ERK/CD44 pathway
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T12%3A28%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=PDRG1%20promotes%20the%20proliferation%20and%20migration%20of%20GBM%20cells%20by%20the%20MEK/ERK/CD44%20pathway&rft.jtitle=Cancer%20science&rft.au=Sun,%20Jinmin&rft.date=2022-02&rft.volume=113&rft.issue=2&rft.spage=500&rft.epage=516&rft.pages=500-516&rft.issn=1347-9032&rft.eissn=1349-7006&rft_id=info:doi/10.1111/cas.15214&rft_dat=%3Cproquest_pubme%3E2625953313%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c4674-a0f0368c9fc3b6174eb3435107c09fe61dc4239ec9633181594bac30f06fde493%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2625953313&rft_id=info:pmid/34812552&rfr_iscdi=true