Quantitative Accuracy and Precision in Multiplexed Single-Cell Proteomics

Single-cell proteomics workflows have considerably improved in sensitivity and reproducibility to characterize as-yet unknown biological phenomena. With the emergence of multiplexed single-cell proteomics, studies increasingly present single-cell measurements in conjunction with an abundant congruen...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2022-02, Vol.94 (5), p.2434-2443
Main Authors: Ctortecka, Claudia, Stejskal, Karel, Krššáková, Gabriela, Mendjan, Sasha, Mechtler, Karl
Format: Article
Language:English
Subjects:
Accuracy
Analytical chemistry
Chemistry
Ion currents
Multiplexing
Proteome
Proteomics
Proteomics - methods
Reproducibility of Results
Workflow
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Summary:Single-cell proteomics workflows have considerably improved in sensitivity and reproducibility to characterize as-yet unknown biological phenomena. With the emergence of multiplexed single-cell proteomics, studies increasingly present single-cell measurements in conjunction with an abundant congruent carrier to improve the precursor selection and enhance identifications. While these extreme carrier spikes are often >100× more abundant than the investigated samples, the total ion current undoubtably increases but the quantitative accuracy possibly is affected. We here focus on narrowly titrated carrier spikes (i.e.,
ISSN:0003-2700
1520-6882
1520-6882
DOI:10.1021/acs.analchem.1c04174