TRIM37 Promotes Pancreatic Cancer Progression through Modulation of Cell Growth, Migration, Invasion, and Tumor Immune Microenvironment

TRIM37 dysregulation has been observed in several cancer types, implicating its possible role in tumorigenesis. However, the role of TRIM37 in pancreatic cancer progression remains unclear. In the present study, we observed that TRIM37 knockdown resulted in reduced proliferation, clonogenicity, migr...

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Bibliographic Details
Published in:International journal of molecular sciences 2022-01, Vol.23 (3), p.1176
Main Authors: Do, Tuyen Thi, Yeh, Chun-Chieh, Wu, Guo-Wei, Hsu, Chia-Chen, Chang, Hung-Chih, Chen, Hui-Chen
Format: Article
Language:English
Subjects:
Animal models
Animals
Apoptosis
Cancer therapies
CD11b antigen
Cell adhesion & migration
Cell growth
Cell Movement
Cell Proliferation
Chemokines
Cloning
Cytokines
Female
Gene Expression Regulation, Neoplastic
Humans
In vivo methods and tests
Localization
Lung cancer
Macrophages
Mice
Mice, Inbred C57BL
Microenvironments
Mutation
Neoplasm Invasiveness
Pancreatic cancer
Pancreatic Neoplasms - genetics
Pancreatic Neoplasms - metabolism
Pancreatic Neoplasms - pathology
Protein expression
Proteins
Regulation
Tripartite Motif Proteins - genetics
Tripartite Motif Proteins - metabolism
Tumor Cells, Cultured
Tumor Microenvironment
Tumorigenesis
Tumors
Ubiquitin-Protein Ligases - genetics
Ubiquitin-Protein Ligases - metabolism
Wound healing
Xenograft Model Antitumor Assays
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Summary:TRIM37 dysregulation has been observed in several cancer types, implicating its possible role in tumorigenesis. However, the role of TRIM37 in pancreatic cancer progression remains unclear. In the present study, we observed that TRIM37 knockdown resulted in reduced proliferation, clonogenicity, migration, and invasion ability of pancreatic cancer cells. Furthermore, an in vivo study using an orthotopic syngeneic animal model further confirmed that reduced expression of TRIM37 in cancer cells suppressed tumor growth in vivo. Moreover, in mice bearing TRIM37 knockdown pancreatic cancer cells, the proportion of CD11b F4/80 MHCII immunosuppressive macrophages was significantly reduced in tumor milieu, which might be due to the regulatory role of TRIM37 in cytokine production by pancreatic cancer cells. Collectively, these findings suggest a key role of TRIM37 in promoting pancreatic cancer progression.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23031176