Characterization of Five Collagenous Biomaterials by SEM Observations, TG-DTA, Collagenase Dissolution Tests and Subcutaneous Implantation Tests

Collagenous biomaterials that are clinically applied in dentistry have dermis-type and membrane-type, both of which are materials for promoting bone and soft tissue formation. The properties of materials supplied with different types could affect their biodegradation periods. The purpose of this stu...

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Bibliographic Details
Published in:Materials 2022-02, Vol.15 (3), p.1155
Main Authors: Hoshi, Miki, Sawada, Tomofumi, Hatakeyama, Wataru, Taira, Masayuki, Hachinohe, Yuki, Takafuji, Kyoko, Kihara, Hidemichi, Takemoto, Shinji, Kondo, Hisatomo
Format: Article
Language:English
Subjects:
Amino acids
Biodegradation
Biomedical materials
Collagen
Contact angle
Dentistry
Differential thermal analysis
Differential thermogravimetric analysis
Dissolution
Extracellular matrix
Implantation
In vivo methods and tests
Manufacturing
Material properties
Membranes
Morphology
Photomicrographs
Porous materials
Proteins
Scanning electron microscopy
Skin
Soft tissues
Spectrum analysis
Stem cells
Surgical implants
Thermogravimetry
Tissue engineering
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Summary:Collagenous biomaterials that are clinically applied in dentistry have dermis-type and membrane-type, both of which are materials for promoting bone and soft tissue formation. The properties of materials supplied with different types could affect their biodegradation periods. The purpose of this study was to characterize five of these products by four different methods: scanning electron microscopy (SEM) observation, thermogravimetry-differential thermal analysis (TG-DTA), 0.01 wt% collagenase dissolution test, and subcutaneous implantation test in vivo. SEM micrographs revealed that both dermis and membranous materials were fibrous and porous. The membranous materials had higher specific derivative thermal gravimetry (DTG) peak temperatures in TG-DTA at around 320 °C, longer collagenase dissolution time ranging from about 300 to 500 min, and more longevity in mice exceeding 9 weeks than the dermis materials. There existed a correlation between the peak temperature in TG-DTA and the collagenase dissolution time. It was considered that higher cross-link degree among collagen fibrils of the membrane-type collagenous materials might account for these phenomena. The experimental protocol and numerical results obtained could be helpful for selection and future development of fibrous collagenous biomaterials in clinical use.
ISSN:1996-1944
1996-1944
DOI:10.3390/ma15031155