Formation of Redox-Active Duroquinone from Vaping of Vitamin E Acetate Contributes to Oxidative Lung Injury

• Published in: Chemical research in toxicology 2022-02, Vol.35 (2), p.254-264
• Main Authors: Canchola, Alexa, Ahmed, C. M. Sabbir, Chen, Kunpeng, Chen, Jin Y, Lin, Ying-Hsuan
• Format: Article
• Language: English
• Subjects: Acetates - chemistry; Acetates - metabolism; Benzoquinones - chemistry; Benzoquinones - metabolism; Electronic Nicotine Delivery Systems; Gas Chromatography-Mass Spectrometry; Humans; Lung Injury - metabolism; Molecular Structure; Oxidation-Reduction; Vaping - metabolism; Vitamin E - chemistry; Vitamin E - metabolism
• ISSN: 0893-228X; 1520-5010
• DOI: 10.1021/acs.chemrestox.1c00309

In late 2019, the outbreak of e-cigarette or vaping-associated lung injuries (EVALIs) in the United States demonstrated to the public the potential health risks of vaping. While studies since the outbreak have identified vitamin E acetate (VEA), a diluent of tetrahydrocannabinol (THC) in vape cartridges, as a potential contributor to lung injuries, the molecular mechanisms through which VEA may cause damage are still unclear. Recent studies have found that the thermal degradation of e-liquids during vaping can result in the formation of products that are more toxic than the parent compounds. In this study, we assessed the role of duroquinone (DQ) in VEA vaping emissions that may act as a mechanism through which VEA vaping causes lung damage. VEA vaping emissions were collected and analyzed for their potential to generate reactive oxygen species (ROS) and induce oxidative stress-associated gene expression in human bronchial epithelial cells (BEAS-2B). Significant ROS generation by VEA vaping emissions was observed in both acellular and cellular systems. Furthermore, exposure to vaping emissions resulted in significant upregulation of NQO1 and HMOX-1 genes in BEAS-2B cells, indicating a strong potential for vaped VEA to cause oxidative damage and acute lung injury; the effects are more profound than exposure to equivalent concentrations of DQ alone. Our findings suggest that there may be synergistic interactions between thermal decomposition products of VEA, highlighting the multifaceted nature of vaping toxicity.