Detection of capsular genotypes of methicillin-resistant Staphylococcus aureus and clonal distribution of the cap5 and cap8 genes in clinical isolates

Up until now, the capsular polysaccharides of Staphylococcus aureus have been classified into 11 types, of which only 2 types 5 and 8; (encoded by the genes cap5 and cap8, respectively) are present in 80–90% of clinically significant strains. The aim of the present study was to detect the capsular g...

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Bibliographic Details
Published in:Archives of microbiology 2022-03, Vol.204 (3), p.186-186, Article 186
Main Authors: Echániz-Aviles, Gabriela, Velazquez-Meza, Maria Elena, Rodríguez-Arvizu, Brian, Carnalla-Barajas, Maria Noemi, Noguerón, Araceli Soto
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Biotechnology
Capsular polysaccharides
Cell Biology
Clinical isolates
Drug resistance
Ecology
Electrophoresis
Electrophoresis, Gel, Pulsed-Field
Gel electrophoresis
Genes
Genotype
Genotypes
Humans
Life Sciences
Methicillin
Methicillin-Resistant Staphylococcus aureus - genetics
Microbial Ecology
Microbial Sensitivity Tests
Microbiology
Multilocus Sequence Typing
Original Paper
Polysaccharides
Public health
Pulsed-field gel electrophoresis
Saccharides
Staphylococcal Infections
Staphylococcus aureus
Staphylococcus aureus - genetics
Staphylococcus infections
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Summary:Up until now, the capsular polysaccharides of Staphylococcus aureus have been classified into 11 types, of which only 2 types 5 and 8; (encoded by the genes cap5 and cap8, respectively) are present in 80–90% of clinically significant strains. The aim of the present study was to detect the capsular genotypes of methicillin-resistant S. aureus (MRSA) clinical isolates and determined their clonal distribution. A total of 262 MRSA clinical isolates from different hospitals in Mexico were analyzed by PCR to determine the genetic characteristics of their capsule expression. Pulsed-field gel electrophoresis and multilocus sequence typing were used to characterize the isolates. The analysis of the capsular genotypes among MRSA isolates showed that 245 isolates (93.5%) contained the cap5 gene, and that the remaining 17 (6.5%) encoded the cap8 gene. The MRSA isolates were grouped into four clonal groups. The identification of the capsular genotypes of clinical isolates of MRSA is important information because potential vaccine formulations against S. aureus involve capsular polysaccharides.
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-022-02793-1