Lung Microenvironments and Disease Progression in Fibrotic Hypersensitivity Pneumonitis

• Published in: American journal of respiratory and critical care medicine 2022-01, Vol.205 (1), p.60-74
• Main Authors: De Sadeleer, Laurens J, McDonough, John E, Schupp, Jonas C, Yan, Xiting, Vanstapel, Arno, Van Herck, Anke, Everaerts, Stephanie, Geudens, Vincent, Sacreas, Annelore, Goos, Tinne, Aelbrecht, Celine, Nawrot, Tim S, Martens, Dries S, Schols, Dominique, Claes, Sandra, Verschakelen, Johny A, Verbeken, Eric K, Ackermann, Maximilian, Decottignies, Anabelle, Mahieu, Manon, Hackett, Tillie-Louise, Hogg, James C, Vanaudenaerde, Bart M, Verleden, Stijn E, Kaminski, Naftali, Wuyts, Wim A
• Format: Article
• Language: English
• Subjects: Adult; Aged; Alveolitis, Extrinsic Allergic - diagnosis; Alveolitis, Extrinsic Allergic - genetics; Alveolitis, Extrinsic Allergic - pathology; Biomarkers; Case-Control Studies; Disease Progression; Female; Fibrosis; Gene Expression Profiling; Genetic Markers; Humans; Linear Models; Lung - pathology; Male; Medical diagnosis; Middle Aged; Original; Pulmonary fibrosis; Reproducibility of Results; Severity of Illness Index; Tomography; Transcription factors; Transcriptome
• ISSN: 1073-449X; 1535-4970
• DOI: 10.1164/rccm.202103-0569OC

Fibrotic hypersensitivity pneumonitis (fHP) is an interstitial lung disease caused by sensitization to an inhaled allergen. To identify the molecular determinants associated with progression of fibrosis. Nine fHP explant lungs and six unused donor lungs (as controls) were systematically sampled (4 samples/lung). According to microcomputed tomography measures, fHP cores were clustered into mild, moderate, and severe fibrosis groups. Gene expression profiles were assessed using weighted gene co-expression network analysis, xCell, gene ontology, and structure enrichment analysis. Gene expression of the prevailing molecular traits was also compared with idiopathic pulmonary fibrosis (IPF). The explant lung findings were evaluated in separate clinical fHP cohorts using tissue, BAL samples, and computed tomography scans. We found six molecular traits that associated with differential lung involvement. In fHP, extracellular matrix and antigen presentation/sensitization transcriptomic signatures characterized lung zones with only mild structural and histological changes, whereas signatures involved in honeycombing and B cells dominated the transcriptome in the most severely affected lung zones. With increasing disease severity, endothelial function was progressively lost, and progressive disruption in normal cellular homeostatic processes emerged. All six were also found in IPF, with largely similar associations with disease microenvironments. The molecular traits correlated with i disease behavior in a separate clinical fHP cohort. We identified six molecular traits that characterize the morphological progression of fHP and associate with clinical behavior. Comparing IPF with fHP, the transcriptome landscape was determined considerably by local disease extent rather than by diagnosis alone.