Na+ and K+ Ions Differently Affect Nucleosome Structure, Stability, and Interactions with Proteins

Inorganic ions are essential factors stabilizing nucleosome structure; however, many aspects of their effects on DNA transactions in chromatin remain unknown. Here, differential effects of K+ and Na+ on the nucleosome structure, stability, and interactions with protein complex FACT (FAcilitates Chro...

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Published in:Microscopy and microanalysis 2022-02, Vol.28 (1), p.243-253
Main Authors: Andreeva, Tatyana V., Maluchenko, Natalya V., Sivkina, Anastasiia L., Chertkov, Oleg V., Valieva, Maria E., Kotova, Elena Y., Kirpichnikov, Mikhail P., Studitsky, Vasily M., Feofanov, Alexey V.
Format: Article
Language:English
Subjects:
Adenosine diphosphate
Biological Applications
Chromatin
Deoxyribonucleic acid
DNA
DNA-directed RNA polymerase
Energy transfer
Experiments
Ions
Microscopy
Nucleosomes
Poly(ADP-ribose)
Poly(ADP-ribose) polymerase
Proteins
Ribose
RNA polymerase
RNA polymerase II
Sodium
Structural stability
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Summary:Inorganic ions are essential factors stabilizing nucleosome structure; however, many aspects of their effects on DNA transactions in chromatin remain unknown. Here, differential effects of K+ and Na+ on the nucleosome structure, stability, and interactions with protein complex FACT (FAcilitates Chromatin Transcription), poly(ADP-ribose) polymerase 1, and RNA polymerase II were studied using primarily single-particle Förster resonance energy transfer microscopy. The maximal stabilizing effect of K+ on a nucleosome structure was observed at ca. 80–150 mM, and it decreased slightly at 40 mM and considerably at >300 mM. The stabilizing effect of Na+ is noticeably lower than that of K+ and progressively decreases at ion concentrations higher than 40 mM. At 150 mM, Na+ ions support more efficient reorganization of nucleosome structure by poly(ADP-ribose) polymerase 1 and ATP-independent uncoiling of nucleosomal DNA by FACT as compared with K+ ions. In contrast, transcription through a nucleosome is nearly insensitive to K+ or Na+ environment. Taken together, the data indicate that K+ environment is more preserving for chromatin structure during various nucleosome transactions than Na+ environment.
ISSN:1431-9276
1435-8115
DOI:10.1017/S1431927621013751