Endophytic fungi from Dongxiang wild rice (Oryza rufipogon Griff.) show diverse catalytic potential for converting glycyrrhizin

Endophytic fungi inhabiting niche environments are novel biocatalyst resources that need to be exploited urgently. In this study, 63 endophytic fungi isolated from Dongxiang wild rice ( Oryza rufipogon Griff.) were tested to assess their potentials to transform glycyrrhizin (GL) into glycyrrhetinic...

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Bibliographic Details
Published in:3 Biotech 2022-03, Vol.12 (3), p.79-79, Article 79
Main Authors: Xiao, Yiwen, Zhang, Zhibin, Liang, Weizhong, Gao, Boliang, Wang, Ya, Chang, Jun, Zhu, Du
Format: Article
Language:English
Subjects:
Agriculture
Biocatalysts
Bioconversion
Bioinformatics
Biomaterials
Biosynthesis
Biotechnology
Biotransformation
Cancer Research
Catalytic converters
Chemistry
Chemistry and Materials Science
Endophytes
Fungi
Glycyrrhizin
Original
Original Article
Oryza rufipogon
Physical characteristics
Sequence analysis
Stem Cells
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Summary:Endophytic fungi inhabiting niche environments are novel biocatalyst resources that need to be exploited urgently. In this study, 63 endophytic fungi isolated from Dongxiang wild rice ( Oryza rufipogon Griff.) were tested to assess their potentials to transform glycyrrhizin (GL) into glycyrrhetinic acid monoglucuronide (GAMG) or glycyrrhetinic acid (GA), of which 12 strains were shown to have β- d -glucuronidase activity. Based on morphological characteristics and rDNA ITS sequence analysis, the strains S59, L138, L55 and R57 with high GL molar conversion rates (55%, 45%, 65% and 89%) were further identified as Microsphaeropsis arundinis S59, Penicillium rubens L138, Aspergillus flavus L55 and Eupenicillium javanicum R57, respectively. These four strains with four different types of GL conversion processes were identified, i.e., (1) GL → GAMG in M. arundinis S59, (2) GL → GAMG and GA in A. flavus L55, (3) GL → GA in P. rubens L138, and (4) GL → GAMG → GA in E. javanicum R57, in which the bioconversion type (4) is reported for the first time. The study not only provided abundant and diverse β- d -glucuronidase resources that can be used for GL bioconversion, especially for GAMG biosynthesis from endophytic fungi, but also expanded our knowledge of potential roles of endophytes as new biocatalysts in biotransformation.
ISSN:2190-572X
2190-5738
DOI:10.1007/s13205-022-03138-x