Peripheral-to-central immune communication at the area postrema glial-barrier following bleomycin-induced sterile lung injury in adult rats

• Published in: Brain, behavior, and immunity behavior, and immunity, 2020-07, Vol.87, p.610-633
• Main Authors: Litvin, David G., Denstaedt, Scott J., Borkowski, Lauren F., Nichols, Nicole L., Dick, Thomas E., Smith, Corey B., Jacono, Frank J.
• Format: Article
• Language: English
• Subjects: Animals; Area Postrema; Bleomycin; Bleomycin - toxicity; Central canal; Circumventricular organs; Communication; Cyclooxygenase; Dorsal motor nucleus of the vagus; Funiculus Separans; Indomethacin; Interleukin-1 Beta; Lung Injury; Neuroglia; Neuroinflammation; Nucleus tractus solitarii; Peripheral-to-central immune communication; Radial-glia; Rats; Rats, Sprague-Dawley; Sterile inflammation; Sterile lung injury
• ISSN: 0889-1591; 1090-2139; 1090-2139
• DOI: 10.1016/j.bbi.2020.02.006

•Sterile lung injury (SLI) activates radial-glia that separate area postrema from CNS.•After SLI, IL-1β & COX-2 localize to radial-glia, which project basolaterally into CNS.•Inhibition of COX-1/2 in the CNS blocks the increases in GFAP and IL-1β after SLI.•Inhibition of COX-1/2 restores sighs that reset the respiratory rhythm. The pathways for peripheral-to-central immune communication (P → C I-comm) following sterile lung injury (SLI) are unknown. SLI evokes systemic and central inflammation, which alters central respiratory control and viscerosensory transmission in the nucleus tractus solitarii (nTS). These functional changes coincide with increased interleukin-1 beta (IL-1β) in the area postrema, a sensory circumventricular organ that connects P → C I-comm to brainstem circuits that control homeostasis. We hypothesize that IL-1β and its downstream transcriptional target, cyclooxygenase-2 (COX-2), mediate P → C I-comm in the nTS. In a rodent model of SLI induced by intratracheal bleomycin (Bleo), the sigh frequency and duration of post-sigh apnea increased in Bleo- compared to saline- treated rats one week after injury. This SLI-dependent change in respiratory control occurred concurrently with augmented IL-1β and COX-2 immunoreactivity (IR) in the funiculus separans (FS), a barrier between the AP and the brainstem. At this barrier, increases in IL-1β and COX-2 IR were confined to processes that stained for glial fibrillary acidic protein (GFAP) and that projected basolaterally to the nTS. Further, FS radial-glia did not express TNF-α or IL-6 following SLI. To test our hypothesis, we blocked central COX-1/2 activity by intracerebroventricular (ICV) infusion of Indomethacin (Ind). Continuous ICV Ind treatment prevented Bleo-dependent increases in GFAP + and IL-1β + IR, and restored characteristics of sighs that reset the rhythm. These data indicate that changes in sighs following SLI depend partially on activation of a central COX-dependent P → C I-comm via radial-glia of the FS.