Oxidative Properties of Blood-Derived Extracellular Vesicles in 15 Patients After Myocardial Infarction

• Published in: Medical science monitor 2022-03, Vol.28, p.e935291-e935291
• Main Authors: Žėkas, Vytautas, Kurg, Reet, Kurg, Kristiina, Bironaitė, Daiva, Radzevičius, Mantas, Karčiauskaitė, Dovilė, Matuzevičienė, Rėda, Kučinskienė, Zita Aušrelė
• Format: Article
• Language: English
• Subjects: Adult; Clinical Research; Exosomes - metabolism; Extracellular Vesicles - metabolism; Female; Follow-Up Studies; Humans; Male; Middle Aged; Myocardial Infarction - metabolism; Myocytes, Cardiac - metabolism; Oxidative Stress; Retrospective Studies
• ISSN: 1643-3750; 1234-1010; 1643-3750
• DOI: 10.12659/MSM.935291

BACKGROUND In this study, we investigated the yield and composition of extracellular vesicles (EVs) derived from 40- to 60-year-old healthy male controls and post-myocardial infarction (post-MI) patients' blood samples and assessed their pro-inflammatory and oxidative-related properties. Our study aimed to determine the EV yield and composition differences between both groups and to find out if there were differences between EV-mediated oxidative stress reactions. MATERIAL AND METHODS Fifteen post-MI patients and 25 healthy individuals were included. EVs were isolated by ultracentrifugation and analyzed using nanotracking analysis (NTA), western blotting and fluorescent flow cytometry (FFC). Oxidative stress (OS) in blood samples was identified by measuring malondialdehyde concentration from serum, while EVs-induced OS was measured in the human vein endothelium cells (HUVEC) using H2DCFDA (2',7'-dichlorodihydrofluorescein diacetate) fluorescence as a marker. RESULTS We found higher EVs concentration in healthy controls than in the post-MI group (7.07±3.1 E+10 ml vs 3.1±1.9 E+10 ml, P