Multimodal high-resolution embryonic imaging with light sheet fluorescence microscopy and optical coherence tomography

A high-resolution imaging system combining optical coherence tomography (OCT) and light sheet fluorescence microscopy (LSFM) was developed. LSFM confined the excitation to only the focal plane, removing the out of plane fluorescence. This enabled imaging a murine embryo with higher speed and specifi...

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Bibliographic Details
Published in:Optics letters 2021-09, Vol.46 (17), p.4180-4183
Main Authors: Khajavi, Behzad, Sun, Ruijiao, Chawla, Harshdeep Singh, Le, Henry H, Singh, Manmohan, Schill, Alexander W, Dickinson, Mary E, Mayerich, David, Larin, Kirill V
Format: Article
Language:English
Subjects:
Animals
Embryos
Fluorescence
Focal plane
High resolution
Image resolution
Laser microscopy
Light sheets
Mice
Microscopy
Microscopy, Fluorescence
Optical Coherence Tomography
Tomography
Tomography, Optical Coherence
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Summary:A high-resolution imaging system combining optical coherence tomography (OCT) and light sheet fluorescence microscopy (LSFM) was developed. LSFM confined the excitation to only the focal plane, removing the out of plane fluorescence. This enabled imaging a murine embryo with higher speed and specificity than traditional fluorescence microscopy. OCT gives information about the structure of the embryo from the same plane illuminated by LSFM. The co-planar OCT and LSFM instrument was capable of performing co-registered functional and structural imaging of mouse embryos simultaneously.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.430202