Circulating microvesicles and exosomes in small cell lung cancer by quantitative proteomics

Early detection of small cell lung cancer (SCLC) crucially demands highly reliable markers. Growing evidence suggests that extracellular vesicles carry tumor cell-specific cargo suitable as protein markers in cancer. Quantitative proteomic profiling of circulating microvesicles and exosomes can be a...

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Bibliographic Details
Published in:Clinical proteomics 2022-01, Vol.19 (1), p.2-2, Article 2
Main Authors: Pedersen, Shona, Jensen, Katrine Papendick, Honoré, Bent, Kristensen, Søren Risom, Pedersen, Camilla Holm, Szejniuk, Weronika Maria, Maltesen, Raluca Georgiana, Falkmer, Ursula
Format: Article
Language:English
Subjects:
Analysis
Biochemistry
Biomarkers
Blood coagulation
Chemotherapy
Chromatography
Clopidogrel
Coagulation
Coagulation factor XIII
Coagulation factors
Complement activation
Complement factor H
Exosomes
Extracellular vesicles
Investigations
Lung cancer
Lung cancer, Small cell
Mass spectrometry
Mass spectroscopy
Medical imaging
Peptides
Plasma
Proteins
Proteomes
Proteomics
Scientific imaging
Small cell lung carcinoma
Statistical analysis
Tumors
Ultracentrifugation
Vesicles
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Summary:Early detection of small cell lung cancer (SCLC) crucially demands highly reliable markers. Growing evidence suggests that extracellular vesicles carry tumor cell-specific cargo suitable as protein markers in cancer. Quantitative proteomic profiling of circulating microvesicles and exosomes can be a high-throughput platform for discovery of novel molecular insights and putative markers. Hence, this study aimed to investigate proteome dynamics of plasma-derived microvesicles and exosomes in newly diagnosed SCLC patients to improve early detection. Plasma-derived microvesicles and exosomes from 24 healthy controls and 24 SCLC patients were isolated from plasma by either high-speed- or ultracentrifugation. Proteins derived from these extracellular vesicles were quantified using label-free mass spectrometry and statistical analysis was carried out aiming at identifying significantly altered protein expressions between SCLC patients and healthy controls. Furthermore, significantly expressed proteins were subjected to functional enrichment analysis to identify biological pathways implicated in SCLC pathogenesis. Based on fold change (FC) ≥ 2 or ≤ 0.5 and AUC ≥ 0.70 (p 
ISSN:1542-6416
1559-0275
DOI:10.1186/s12014-021-09339-5