Detection of partial and/or complete Y chromosome microdeletions of azoospermia factor a (AZFa) sub‐region in infertile Iraqi patients with azoospermia and severe oligozoospermia

Background This study aimed to analyze the incidence of azoospermia factor a (AZFa) microdeletions in the Y chromosome and their association with male infertility in a population with azoospermia and severe oligozoospermia from Iraq. Methods A total of 75 infertile Iraqi males and 25 healthy control...

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Bibliographic Details
Published in:Journal of clinical laboratory analysis 2022-03, Vol.36 (3), p.e24272-n/a
Main Authors: S. Al‐Ouqaili, Mushtak T., Al‐Ani, Sahar K., Alaany, Rehab, Al‐Qaisi, Mohammed N.
Format: Article
Language:English
Subjects:
Azoospermia - epidemiology
Azoospermia - genetics
Chromosome Deletion
Chromosomes, Human, Y
complete AZF microdeletion
Ethics
Genes
Genomes
Health care
Humans
Infertility
Infertility, Male - genetics
Iraq - epidemiology
Male
male infertility
Males
Oligospermia - epidemiology
Oligospermia - genetics
Oligozoospermia
partial AZFa/ microdeletion
Polymerase chain reaction
Sex Chromosome Aberrations
Sex Chromosome Disorders of Sex Development
Sperm
Spermatogenesis
Vitamin B 12 - analogs & derivatives
Y chromosomes
Y/chromosome/microdeletion
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Summary:Background This study aimed to analyze the incidence of azoospermia factor a (AZFa) microdeletions in the Y chromosome and their association with male infertility in a population with azoospermia and severe oligozoospermia from Iraq. Methods A total of 75 infertile Iraqi males and 25 healthy controls were included in this study. The semen analysis was performed to determine the azoospermia, severe oligozoospermia, or normal cases. The AZFa microdeletions were investigated using the real‐time polymerase chain reaction (real‐time PCR). Then, AZFa sub‐region deletions were investigated by a conventional PCR. Results In total, 40 men with azoospermia and 35 men with severe oligozoospermia were selected. Out of 75 infertile males, 46 (61.3%) individuals had AZFa microdeletions, of whom 32 (69.6%) had partial deletion, while 14 (30.4%) males had complete deletion using real‐time PCR. The frequency of microdeletions was significantly different between the infertile and control group (p‐value 
ISSN:0887-8013
1098-2825
DOI:10.1002/jcla.24272