Alternative Brain Slice-on-a-Chip for Organotypic Culture and Effective Fluorescence Injection Testing

Mouse brain slices are one of the most common models to study brain development and functioning, increasing the number of study models that integrate microfluidic systems for hippocampal slice cultures. This report presents an alternative brain slice-on-a-chip, integrating an injection system inside...

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Bibliographic Details
Published in:International journal of molecular sciences 2022-02, Vol.23 (5), p.2549
Main Authors: Herreros, Pedro, Tapia-González, Silvia, Sánchez-Olivares, Laura, Laguna Heras, María Fe, Holgado, Miguel
Format: Article
Language:English
Subjects:
Animals
Biological properties
Bioreactors
Brain
Brain architecture
Brain slice preparation
Cell culture
Contamination
Fluorescence
Fluorescent dyes
Fluorescent indicators
Hippocampus
Injection
Lab-On-A-Chip Devices
Labeling
Mice
Microfluidics
Organ Culture Techniques
Reagents
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Summary:Mouse brain slices are one of the most common models to study brain development and functioning, increasing the number of study models that integrate microfluidic systems for hippocampal slice cultures. This report presents an alternative brain slice-on-a-chip, integrating an injection system inside the chip to dispense a fluorescent dye for long-term monitoring. Hippocampal slices have been cultured inside these chips, observing fluorescence signals from living cells, maintaining the cytoarchitecture of the slices. Having fluorescence images of biological samples inside the chip demonstrates the effectiveness of the staining process using the injection method avoiding leaks or biological contamination. The technology developed in this study presents a significant improvement in the local administration of reagents within a brain slice-on-a-chip system, which could be a suitable option for organotypic cultures in a microfluidic chip acting as a highly effective bioreactor.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23052549