Fragments of the Fab-3 and Fab-4 Boundaries of the Drosophila melanogaster Bithorax Complex That Include CTCF Sites Are not Effective Insulators

The segment-specific regulatory domains of the Bithorax complex (BX-C), which consists of three homeotic genes Ubx, abd-A and Abd-B , are separated by boundaries that function as insulators. Most of the boundaries contain binding sites for the architectural protein CTCF, which is conserved for highe...

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Bibliographic Details
Published in:Doklady. Biochemistry and biophysics 2022-02, Vol.502 (1), p.21-24
Main Authors: Kyrchanova, O. V., Postika, N. Y., Sokolov, V. V., Georgiev, P. G.
Format: Article
Language:English
Subjects:
Animals
Binding Sites
Biochemistry
Biochemistry, Biophysics, and Molecular Biology
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Boundaries
CCCTC-Binding Factor - genetics
CCCTC-Binding Factor - metabolism
Drosophila melanogaster - genetics
Drosophila melanogaster - metabolism
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Genes, Homeobox
Homeodomain Proteins - genetics
Homeodomain Proteins - metabolism
Insulator Elements
Life Sciences
Molecular Biology
Transcription Factors - metabolism
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Summary:The segment-specific regulatory domains of the Bithorax complex (BX-C), which consists of three homeotic genes Ubx, abd-A and Abd-B , are separated by boundaries that function as insulators. Most of the boundaries contain binding sites for the architectural protein CTCF, which is conserved for higher eukaryotes. As was shown previously, the CTCF sites determine the insulator activity of the boundaries of the Abd-B regulatory region. In this study, it was shown that fragments of the Fab-3 and Fab-4 boundaries of the abd-A regulatory region, containing CTCF binding sites, are not effective insulators.
ISSN:1607-6729
1608-3091
DOI:10.1134/S1607672922010069