Loading…

Metabolic fingerprinting for discrimination of DNA-authenticated Atractylodes plants using 1H NMR spectroscopy

Identifying different species of the genus Atractylodes which are commonly used in Chinese and Japanese traditional medicine, using chromatographic approaches can be difficult. 1 H NMR metabolic profiling of DNA-authenticated, archived rhizomes of the genus Atractylodes was performed for genetic and...

Full description

Saved in:
Bibliographic Details
Published in:Journal of natural medicines 2021, Vol.75 (3), p.475-488
Main Authors: Shirahata, Tatsuya, Ishikawa, Hiroshi, Kudo, Teruhisa, Takada, Yumiko, Hoshino, Azusa, Taga, Yui, Minakuchi, Yusaku, Hasegawa, Tomoko, Horiguchi, Rina, Hirayama, Takehiro, Konishi, Takahiro, Takemoto, Hiroaki, Sato, Noriko, Aragane, Masako, Oikawa, Tetsuro, Odaguchi, Hiroshi, Hanawa, Toshihiko, Kodaira, Eiichi, Fukuda, Tatsuo, Kobayashi, Yoshinori
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Identifying different species of the genus Atractylodes which are commonly used in Chinese and Japanese traditional medicine, using chromatographic approaches can be difficult. 1 H NMR metabolic profiling of DNA-authenticated, archived rhizomes of the genus Atractylodes was performed for genetic and chemical evaluation. The ITS region of the nuclear rDNA was sequenced for five species, A. japonica , A. macrocephala , A. lancea , A. chinensis , and A. koreana . Our samples had nucleotide sequences as previously reported, except that part of the A. lancea cultivated in Japan had a type 5, hybrid DNA sequence. Principal component analysis (PCA) using 1 H NMR spectra of extracts with two solvent systems (CD 3 OD, CDCl 3 ) was performed. When CDCl 3 extracts were utilized, the chemometric analysis enabled the identification and classification of Atractylodes species according to their composition of major sesquiterpene compounds. The 1 H NMR spectra using CD 3 OD contained confounding sugar peaks. PCA removal of these peaks gave the same result as that obtained using CDCl 3 and allowed species distinction. Such chemometric methods with multivariate analysis of NMR spectra will be useful for the discrimination of plant species, without specifying the index components and quantitative analysis on multi-components. Graphic abstract
ISSN:1340-3443
1861-0293
DOI:10.1007/s11418-020-01471-0