Compact RNA editors with small Cas13 proteins

CRISPR–Cas13 systems have been developed for precise RNA editing, and can potentially be used therapeutically when temporary changes are desirable or when DNA editing is challenging. We have identified and characterized an ultrasmall family of Cas13b proteins—Cas13bt—that can mediate mammalian trans...

Full description

Saved in:
Bibliographic Details
Published in:Nature biotechnology 2022-02, Vol.40 (2), p.194-197
Main Authors: Kannan, Soumya, Altae-Tran, Han, Jin, Xin, Madigan, Victoria J., Oshiro, Rachel, Makarova, Kira S., Koonin, Eugene V., Zhang, Feng
Format: Article
Language:English
Subjects:
631/337
631/61
Adenosine
Adenosine - genetics
Adenosine deaminase
Adenosine Deaminase - genetics
Agriculture
Animals
Bioinformatics
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Brief Communication
CRISPR
CRISPR-Cas Systems - genetics
Cytosine
Cytosine deaminase
Editing
Gene Editing
Life Sciences
Mammals - genetics
Packaging
Proteins
Ribonucleic acid
RNA
RNA - genetics
RNA editing
RNA Editing - genetics
Transcription
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:CRISPR–Cas13 systems have been developed for precise RNA editing, and can potentially be used therapeutically when temporary changes are desirable or when DNA editing is challenging. We have identified and characterized an ultrasmall family of Cas13b proteins—Cas13bt—that can mediate mammalian transcript knockdown. We have engineered compact variants of REPAIR and RESCUE RNA editors by functionalizing Cas13bt with adenosine and cytosine deaminase domains, and demonstrated packaging of the editors within a single adeno-associated virus. Compact Cas13 proteins are used to design RNA editors that fit into a single adeno-associated virus.
ISSN:1087-0156
1546-1696
DOI:10.1038/s41587-021-01030-2