Identification of Trueperella bernardiae isolated from peking ducks (Anas platyrhynchos domesticus) by phenotypical and genotypical investigations and by a newly developed loop-mediated isothermal amplification (LAMP) assay

• Published in: Folia microbiologica 2022-04, Vol.67 (2), p.277-284
• Main Authors: Ahmed, Marwa F. E., Alssahen, Mazen, Lämmler, Christoph, Köhler, Bernd, Metzner, Martin, Plötz, Madeleine, Abdulmawjood, Amir
• Format: Article
• Language: English
• Subjects: Actinomycetaceae; Animals; Applied Microbiology; Aquatic birds; Arcanobacterium - genetics; Assaying; Beijing; Biomedical and Life Sciences; Ducks; Ducks - genetics; Environmental Engineering/Biotechnology; Farms; Gene amplification; Gene sequencing; Glyceraldehyde-3-phosphate dehydrogenase; Immunology; Ionization; Ions; Life Sciences; Mass spectrometry; Mass spectroscopy; Microbiology; Molecular Diagnostic Techniques; Nucleic Acid Amplification Techniques; Original; Original Article; RNA, Ribosomal, 16S - genetics; rRNA 16S; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Superoxide dismutase; Waterfowl
• ISSN: 0015-5632; 1874-9356
• DOI: 10.1007/s12223-021-00927-4

Trueperella ( T. ) bernardiae is a well-known bacterial pathogen in infections of humans, rarely in animals. In the present study, five T . bernardiae isolates, isolated from five Peking ducks of four different farms, were identified by phenotypic properties, by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, and genotypically by sequencing the 16S ribosomal RNA (rRNA) gene, the superoxide dismutase A encoding gene sodA , and the glyceraldehyde-3-phosphate dehydrogenase encoding gene gap . In addition, the T . bernardiae isolates could be identified with a newly developed loop-mediated isothermal amplification (LAMP) assay based on the gyrase encoding housekeeping gene gyrA . All these tests clearly identified the T . bernardiae isolates to the species level. However, the detection of the specific gene gyrA with the newly designed LAMP assay appeared with a high sensitivity and specificity, and could help to identify this bacterial species in human and animal infections in future. The importance of the T . bernardiae isolates for the clinical condition of the ducks and for the problems at farm level remains unclear.