Effects of passive and active leg movements to interrupt sitting in mild hypercapnia on cardiovascular function in healthy adults

• Published in: Journal of applied physiology (1985) 2022-03, Vol.132 (3), p.874-887
• Main Authors: Park, Song-Young, Wooden, TeSean K, Pekas, Elizabeth J, Anderson, Cody P, Yadav, Santosh K, Slivka, Dustin R, Layec, Gwenael
• Format: Article
• Language: English
• Subjects: Adult; Adults; Brachial Artery; Carbon dioxide; Cardiovascular system; Endothelin 1; Endothelins; Endothelium, Vascular - physiology; Frequencies; Heart rate; Humans; Hypercapnia; Leg; Lower Extremity - blood supply; Microvasculature; Muscle contraction; Muscles; Muscular function; Regional Blood Flow - physiology; Shear rate; Skeletal muscle; Vasoactive agents; Vasodilation - physiology
• ISSN: 8750-7587; 1522-1601
• DOI: 10.1152/japplphysiol.00799.2021

Prolonged sitting in a mild hypercapnic environment impairs peripheral vascular function. The effects of sitting interruptions using passive or active skeletal muscle contractions are still unclear. Therefore, we sought to examine the vascular effects of brief periods (2 min every half hour) of passive and active lower limb movement to interrupt prolonged sitting with mild hypercapnia in adults. Fourteen healthy adults (24 ± 2 yr) participated in three experimental visits sitting for 2.5 h in a mild hypercapnic environment (CO  = 1,500 ppm): control (CON, no limb movement), passive lower limb movement (PASS), and active lower limb movement (ACT) during sitting. At all visits, brachial and popliteal artery flow-mediated dilation (FMD), microvascular function, plasmatic levels of nitrate/nitrite and endothelin-1, and heart rate variability were assessed before and after sitting. Brachial and popliteal artery FMDs were reduced in CON and PASS ( < 0.05) but were preserved ( > 0.05) in ACT. Microvascular function was blunted in CON ( < 0.05) but was preserved in PASS and ACT ( > 0.05). In addition, total plasma nitrate/nitrite was preserved in ACT ( > 0.05) but was reduced in CON and PASS ( < 0.05), and endothelin-1 levels were decreased in ACT ( < 0.05). Both passive and active movement induced a greater ratio between the low-frequency and high-frequency bands for heart rate variability ( < 0.05). For the first time, to our knowledge, we found that brief periods of passive leg movement can preserve microvascular function, but that an intervention that elicits larger increases in shear rate, such as low-intensity exercise, is required to fully protect both macrovascular and microvascular function and circulating vasoactive substance balance. Passive leg movement could not preserve macrovascular endothelial function, whereas active leg movement could protect endothelial function. Attenuated microvascular function can be salvaged by passive movement and active movement. Preservation of macrovascular hemodynamics and plasma total nitrate/nitrite and endothelin-1 during prolonged sitting requires active movement. These findings dissociate the impacts induced by mechanical stress (passive movement) from the change in metabolism (active movement) on the vasculature during prolonged sitting in a mild hypercapnic environment.