Persistence of Endogenous SARS-CoV‑2 and Pepper Mild Mottle Virus RNA in Wastewater-Settled Solids

Limited information is available on the decay rate of endogenous SARS-CoV-2 and pepper mild mottle virus (PMMoV) RNA in wastewater and primary settled solids, potentially limiting an understanding of how transit or holding times within wastewater infrastructure might impact RNA measurements and thei...

Full description

Saved in:
Bibliographic Details
Published in:ACS ES&T water 2022-11, Vol.2 (11), p.1944-1952
Main Authors: Roldan-Hernandez, Laura, Graham, Katherine E., Duong, Dorothea, Boehm, Alexandria B.
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Limited information is available on the decay rate of endogenous SARS-CoV-2 and pepper mild mottle virus (PMMoV) RNA in wastewater and primary settled solids, potentially limiting an understanding of how transit or holding times within wastewater infrastructure might impact RNA measurements and their relationship to community COVID-19 infections. In this study, primary settled solids samples were collected from two wastewater treatment plants in the San Francisco Bay Area. Samples were thoroughly mixed, aliquoted into subsamples, and stored at 4, 22, and 37 °C for 10 days. The concentrations of SARS-CoV-2 (N1 and N2 targets) and PMMoV RNA were measured using an RT-ddPCR. Limited decay (
ISSN:2690-0637
2690-0637
DOI:10.1021/acsestwater.2c00003