Transcriptomic and Metabolomic Analyses Provide Insights into the Growth and Development Advantages of Triploid Apostichopus japonicus

• Published in: Marine biotechnology (New York, N.Y.) N.Y.), 2022-03, Vol.24 (1), p.151-162
• Main Authors: Xie, Jiahui, Sun, Yi, Cao, Yue, Han, Lingshu, Li, Yuanxin, Ding, Beichen, Gao, Chuang, Hao, Pengfei, Jin, Xin, Chang, Yaqing, Song, Jian, Yin, Donghong, Ding, Jun
• Format: Article
• Language: English
• Subjects: Aestivation; Animals; Apostichopus japonicus; Aquaculture; ATP; Biological activity; Biological data; Biological Phenomena; Biomedical and Life Sciences; Body length; Body weight; Breeding; Cyclin-dependent kinase; Cyclin-dependent kinase 2; Cyclin-dependent kinases; Cytoplasm; Diploids; Encyclopaedias; Encyclopedias; Energy storage; Engineering; Enrichment; Freshwater & Marine Ecology; Gene Expression Profiling; Genes; Genetic improvement; Genetic resources; Genomes; Growth; Growth and Development; Immunoregulation; Kinases; Life Sciences; Metabolic pathways; Metabolism; Metabolites; Metabolomics; Microbiology; Nocodazole; Original; Original Article; Oxidation; Polyploidy; Ribosomes; Stichopus - genetics; Transcriptome; Transcriptomes; Transcriptomics; Triploidy; Zoology
• ISSN: 1436-2228; 1436-2236
• DOI: 10.1007/s10126-022-10093-4

Polyploid breeding is widely used in aquaculture as an important area of new research. We have previously grown Apostichopus japonicus triploids with a growth advantage. The body length, body weight, and aestivation time of triploid and diploid A. japonicus were measured in this study, and the transcriptome and metabolome were used to examine the growth advantage of triploids A. japonicus . The results showed that the proportion of triploid A. japonicus with a body length of 6–12 cm and 12–18 cm was significantly higher than that of diploid A. japonicus , and triploid A. japonicus had a shorter aestivation time (39 days) than diploid (63 days). We discovered 3296 differentially expressed genes (DEGs); 13 DEGs (for example, cyclin-dependent kinase 2 ) related to growth advantage, immune regulation, and energy storage were screened as potential candidates. According to Gene Ontology (GO) enrichment analysis, DEGs were significantly enriched in the cytoplasm (cellular component), ATP binding process (molecular function), oxidation–reduction process (biological process), and other pathways. According to the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment data, DEGs were significantly enriched in ribosome production and other areas. We discovered 414 significant differential metabolites (SDMs), with 11 important SDMs (for example, nocodazole) linked to a growth advantage. SDMs are significantly enriched in metabolic pathways, as well as other pathways, according to the KEGG enrichment results. According to a combined transcriptome and metabolome analysis, 6 DEGs have regulatory relationships with 11 SDMs, which act on 11 metabolic pathways together. Our results further enrich the biological data of triploid A. japonicus and provide useful resources for genetic improvement of this species.