Comparative transcriptome analysis revealed the molecular mechanism of the effect of light intensity on the accumulation of rhynchophylline and isorhynchophylline in Uncaria rhynchophylla

• Published in: Physiology and molecular biology of plants 2022-02, Vol.28 (2), p.315-331
• Main Authors: Wang, Xiao-Hong, Li, Xue, Qiang, Wei, Yu, Xiao-Song, Zheng, Hao-Jie, Zhang, Ming-Sheng
• Format: Article
• Language: English
• Subjects: Accumulation; Alzheimer's disease; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Biosynthesis; Cell Biology; Cultivation techniques; Environmental factors; Gene expression; Life Sciences; Light; Light intensity; Luminous intensity; Medicinal plants; Neurodegenerative diseases; Plant Physiology; Plant Sciences; Research Article; Shade; Signal transduction; Transcription factors; Transcriptomes; Uncaria rhynchophylla
• ISSN: 0971-5894; 0974-0430
• DOI: 10.1007/s12298-022-01142-2

Rhynchophylline (RIN) and isorhynchophylline (IRN), the main medicinal components in plant Uncaria rhynchophylla , have potential effects on Alzheimer’s disease. Understanding the influence of environmental factors, especially light intensity, on the production of these active ingredients will help to improve cultivation techniques. Compared with the 100% light intensity (CK), the contents of RIN and IRN in U. rhynchophylla leaves significantly increased at 20% light intensity (HS) after 7 and 21 days. Short-term shading (21d) changed some morphological indicators of U. rhynchophylla , but did not affect its biomass. Transcriptome profile analysis was performed on data from two groups (7 and 21 days) of CK and HS samples and yielded 79,817 unigenes with an average length of 1023 bp. Concurrently, 2391 and 2136 differentially expressed genes were identified in the transcriptome data for, respectively, 7 and 21 days of shade treatment. Notably, unigenes known to be involved upstream in the biosynthesis of RIN and IRN, such as G8O , IO , 7-DLGT , LAMT , TDC , and STR, were mostly upregulated. In addition, 1065 putative transcription factors (TFs) were identified and grouped into 55 TF families; 26 TFs showed differential expression in the shade treatment after 7 and 21 days. HY5 and PIFs, two important TFs of the light signaling pathway, also showed differential expression. This study provides insight into how gene expression was affected by light intensity during RIN and IRN accumulation in U. rhynchophylla .