Mercury biomonitoring in German adults using volumetric absorptive microsampling

Mercury (Hg) is a global pollutant and a danger to human health. Human biomonitoring of Hg using a dried blood matrix instead of venous blood sampling for exposure assessment is of growing interest. This study aims to develop, validate, and evaluate the application of volumetric absorptive microsamp...

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Bibliographic Details
Published in:Environmental monitoring and assessment 2022-04, Vol.194 (4), p.315-315, Article 315
Main Authors: Koutsimpani-Wagner, Anastasia, Quartucci, Caroline, Rooney, James P. K., Bose-O’Reilly, Stephan, Rakete, Stefan
Format: Article
Language:English
Subjects:
Absorptivity
Accuracy
Adult
Analysis
Atmospheric Protection/Air Quality Control/Air Pollution
Biological Monitoring
Biomonitoring
Blood
Blood Specimen Collection - methods
Desiccants
Desiccators
Dried Blood Spot Testing - methods
Drying
Earth and Environmental Science
Ecology
Ecotoxicology
Environment
Environmental Management
Environmental Monitoring
Environmental science
Evaluation
Humans
Hydrochloric acid
Laboratories
Mercury
Monitoring/Environmental Analysis
Pollutants
Quantitation
Reference materials
Sampling
Stability analysis
Storage conditions
Tandem Mass Spectrometry - methods
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Summary:Mercury (Hg) is a global pollutant and a danger to human health. Human biomonitoring of Hg using a dried blood matrix instead of venous blood sampling for exposure assessment is of growing interest. This study aims to develop, validate, and evaluate the application of volumetric absorptive microsampling (VAMS) for Hg biomonitoring in humans. Sampling, drying, and storage conditions were evaluated during method development. Storage in pre-cleaned glass vials after drying for 2 h in a desiccator ensured analyte stability for at least 4 weeks. Sixty-eight paired capillary VAMS and venous blood samples from volunteers in Munich, Germany, were used for method validation. Hg levels in VAMS and venous blood samples were determined by direct mercury analysis. The limits of detection and quantitation for VAMS were 0.18 and 0.61 µg/l, respectively. However, sensitivity could be improved by using two microsamples for analysis instead of one. Hg levels in VAMS samples correlated very well with Hg levels in venous blood samples (R 2  = 0.958). Furthermore, VAMS showed a high accuracy (median recovery: 117%) and precision (median relative standard deviation: 8.7%), especially for Hg concentrations above 1.0 µg/l. In fact, accuracy and precision of VAMS improved with increasing Hg concentrations. In conclusion, VAMS in combination with direct mercury analysis is an accurate and viable alternative for human biomonitoring of Hg. Graphical abstract
ISSN:0167-6369
1573-2959
DOI:10.1007/s10661-022-09962-1