Femtosecond laser oocyte enucleation as a low-invasive and effective method of recipient cytoplast preparation

Recipient cytoplast preparation, commonly performed by DNA aspiration with a needle, inevitably leads to the loss of reprogramming factors. As an alternative to the traditional enucleation technique, femtosecond laser enucleation can eliminate DNA effectively without loss of reprogramming factors an...

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Bibliographic Details
Published in:Biomedical optics express 2022-03, Vol.13 (3), p.1447-1456
Main Authors: Osychenko, Alina A, Zalessky, Alexandr D, Tochilo, Uliana A, Martirosyan, David Yu, Silaeva, Yulia Yu, Nadtochenko, Victor A
Format: Article
Language:English
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Summary:Recipient cytoplast preparation, commonly performed by DNA aspiration with a needle, inevitably leads to the loss of reprogramming factors. As an alternative to the traditional enucleation technique, femtosecond laser enucleation can eliminate DNA effectively without loss of reprogramming factors and without oocyte puncturing. In this work we have performed oocyte enucleation by destructing the metaphase plate using a 795 nm femtosecond laser. The disability of the enucleated oocytes to develop after the parthenogenetic activation, as well as the lack of DNA staining luminescence, strongly confirms the efficiency of the femtosecond laser enucleation. The parthenogenetic development of oocytes after the cytoplasm treatment suggests a low-invasive effect of the laser enucleation technique.
ISSN:2156-7085
2156-7085
DOI:10.1364/BOE.449523