Well-Plate μFASP for Proteomic Analysis of Single Pancreatic Islets

Filter-aided sample preparation (FASP) is widely used in bottom-up proteomics for tryptic digestion. However, the sample recovery yield of this method is limited by the amount of the starting material. While ∼100 ng of digested protein is sufficient for thorough protein identification, proteomic inf...

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Bibliographic Details
Published in:Journal of proteome research 2022-04, Vol.21 (4), p.1167-1174
Main Authors: Sandbaumhüter, Friederike A, Nezhyva, Mariya, Eriksson, Olle, Engberg, Adam, Kreuger, Johan, Andrén, Per E, Jansson, Erik T
Format: Article
Language:English
Subjects:
Chromatography, Liquid - methods
Humans
Islets of Langerhans - chemistry
Mass Spectrometry
Proteins - analysis
Proteomics - methods
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Summary:Filter-aided sample preparation (FASP) is widely used in bottom-up proteomics for tryptic digestion. However, the sample recovery yield of this method is limited by the amount of the starting material. While ∼100 ng of digested protein is sufficient for thorough protein identification, proteomic information gets lost with a protein content
ISSN:1535-3893
1535-3907
DOI:10.1021/acs.jproteome.2c00047