Multidrug-Resistant Bacteria and Enterobacteriaceae Count in Abattoir Wastes and Its Receiving Waters in Limbe Municipality, Cameroon: Public Health Implications

• Published in: BioMed research international 2022-03, Vol.2022, p.9977371-11
• Main Authors: Esemu, Seraphine Nkie, Aka, Tendongmo Kinsley, Kfusi, Achah Jerome, Ndip, Roland Ndip, Ndip, Lucy Mande
• Format: Article
• Language: English
• Subjects: Abattoirs; Amikacin; Ampicillin; Anti-Bacterial Agents - pharmacology; Antibiotic resistance; Antibiotics; Antiinfectives and antibacterials; Bacteria; Biomedical research; Cameroon; Ciprofloxacin; Drug resistance; Drug resistance in microorganisms; E coli; Enterobacter; Enterobacteriaceae; Enterobacteriaceae infections; Environmental aspects; Escherichia coli; Escherichia coli Infections; Health aspects; Health risks; Humans; Hygiene; Identification and classification; Kanamycin; Laboratories; Meat; Multidrug resistance; Multidrug resistant organisms; Pathogens; Polymerase chain reaction; Public Health; Receiving waters; Risk factors; Salmonella; Sanitation; Shigella; Slabs; Slaughter; Slaughtering and slaughter-houses; Staphylococcal Infections - microbiology; Staphylococcus aureus; Statistical analysis; Statistical methods; Vancomycin; Waste disposal; Waste management; Wastes; Water; Water pollution
• ISSN: 2314-6133; 2314-6141; 2314-6141
• DOI: 10.1155/2022/9977371

The release of untreated wastes from abattoirs into the environment and nearby water bodies poses a significant threat to public health. Such litters may contain pathogens, including antibiotic-resistant bacteria. This study investigated 80 samples collected from butchering tables, slaughter slabs, meat rinsing points, and abattoir wastes receiving water from two abattoirs (A and B). Total Enterobacteriaceae count (TEC) for each sample was determined, and Escherichia coli (E. coli), Salmonella spp., Shigella spp., and Staphylococcus aureus (S. aureus) were isolated and identified. Antimicrobial susceptibility testing was done on all bacterial isolates against nine locally used antibiotics. Overall, 118 bacterial isolates, comprising E. coli (42.5%), Salmonella spp. (27.5%), Shigella spp. (37.5%), and S. aureus (40.0%), were recovered. Of the 118 bacterial isolates, 104 (88.1%) were multidrug-resistant, including 58 (55.8%) from abattoir A and 46 (44.2%) from abattoir B; however, this difference was not statistically significant (p=0.6837). Of the 32 S. aureus isolates, 29 (90.6%) were multidrug-resistant. All S. aureus were 100% sensitive to vancomycin, kanamycin, and amikacin. Similarly, 31 (91.2%) of the 34 E. coli isolates recovered in this study were multidrug-resistant. Salmonella spp. and Shigella spp. also showed high levels of multidrug resistance corresponding to 81.8% and 86.7%, respectively. All isolates of E. coli, Salmonella, and Shigella were 100% resistant to ampicillin and 100% sensitive to ciprofloxacin. Minimum and maximum mean values for TEC were 3.62-5.83 log CFU/mL for abattoir A and 4.08–5.56 log CFU/mL for abattoir B. The highest and lowest TEC counts were from slaughter slab and upstream water, respectively, in each abattoir. Our results indicate a predominance of multidrug-resistant bacteria in abattoir wastes and their receiving waters in the study sites. Hence, we recommend the treatment of abattoir wastes before disposal and improved hygiene and sanitation practices to enhance public health.