Highly mercury-resistant strains from different Colombian Amazon ecosystems affected by artisanal gold mining activities

• Published in: Applied microbiology and biotechnology 2022-04, Vol.106 (7), p.2775-2793
• Main Authors: Cardona, Gladys Inés, Escobar, María Camila, Acosta-González, Alejandro, Marín, Patricia, Marqués, Silvia
• Format: Article
• Language: English
• Subjects: Antibiotics; Bacillus; Bacteria; Bacteria - genetics; Biomedical and Life Sciences; Bioremediation; Biotechnology; Chemical reduction; Colombia; Conserved sequence; Ecosystem; Environmental aspects; Environmental Biotechnology; Forest soils; Fungi; Gene transfer; Genera; Gold; Gold mines & mining; Gold mines and mining; Horizontal transfer; Humans; Life Sciences; Mercuric chloride; Mercury; Mercury (metal); Mercury - analysis; Mercury pollution; Microbial Genetics and Genomics; Microbiology; Minimum inhibitory concentration; Mining; Physiological aspects; Pollution levels; Pseudomonas; River sediments; Sediment samplers; Sediments; Soil; Soil contamination; Strains (organisms); Underground mining; Yeasts
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-022-11860-y

Two sites of the Colombian Amazon region with different levels of human intervention and mercury pollution were selected for the collection of samples of river and lake water, sediments, and associated forest soils. The Tarapacá region, affected mainly by barrage mining, showed low mercury concentrations, whilst in the Taraira region, affected by underground mining, there were several points with high mercury pollution levels. A collection of 72 bacterial and 10 yeast strains with different levels of mercury resistance was isolated and characterized. Most of the highly resistant bacterial strains (MIC > 40 mg L −1 HgCl 2 ) were isolated from soil and sediment samples and belonged to either Pseudomonas (60%) or Bacillus (20%). Most of highly resistant bacterial strains were positive for the presence of the merA gene, suggesting an active mercury resistance mechanism. This was confirmed in the two most resistant strains, Pseudomonas sp. TP30 and Burkholderia contaminans TR100 (MIC = 64 and 71 mg L −1 HgCl 2 , respectively), which in the presence of increasing mercury concentrations expressed the merA gene at increasing levels, concomitant with a significant mercury reduction activity. Analysis of the MerA sequences present in the different isolates suggested a high gene conservation within the taxonomic groups but also several horizontal gene transfer events between taxonomically distant genera. We also observed a positive correspondence between the presence of the merA gene and the number of antibiotics to which the strains were resistant to. The most resistant strains are good candidates for future applications in the bioremediation of mercury-contaminated sites in the Amazon. Key points • Amazon sediments affected by underground gold mining have higher Hg levels. • Highly Hg-resistant isolates belonged to Pseudomonas and Bacillus genera. • TR100 and TP30 strains showed remediation potential to be used in the Amazon region. Graphical abstract