Developing reverse genetics systems of northern cereal mosaic virus to reveal superinfection exclusion of two cytorhabdoviruses in barley plants

Recently, reverse genetics systems of plant negative‐stranded RNA (NSR) viruses have been developed to study virus–host interactions. Nonetheless, genetic rescue of plant NSR viruses in both insect vectors and monocot plants is very limited. Northern cereal mosaic virus (NCMV), a plant cytorhabdovir...

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Published in:Molecular plant pathology 2022-05, Vol.23 (5), p.749-756
Main Authors: Fang, Xiao‐Dong, Qiao, Ji‐Hui, Zang, Ying, Gao, Qiang, Xu, Wen‐Ya, Gao, Dong‐Min, Yang, Yi‐Zhou, Xie, Liang, Wang, Ying, Wang, Xian‐Bing
Format: Article
Language:English
Subjects:
Barley
Cloning
cytorhabdovirus
Fluorescence
Genes
Genetics
Genomes
Green fluorescent protein
Infections
Insects
Insertion
Kinases
monocot plants
NCMV
Plant diseases
plant negative‐stranded RNA viruses
Plant viruses
Plasmids
Proteins
Red fluorescent protein
reverse genetics systems
RNA viruses
Short Communication
Superinfection
superinfection exclusion (SIE)
Tobacco
Vectors
Viruses
West Nile virus
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Summary:Recently, reverse genetics systems of plant negative‐stranded RNA (NSR) viruses have been developed to study virus–host interactions. Nonetheless, genetic rescue of plant NSR viruses in both insect vectors and monocot plants is very limited. Northern cereal mosaic virus (NCMV), a plant cytorhabdovirus, causes severe diseases in cereal plants through transmission by the small brown planthopper (SBPH, Laodelphax striatellus) in a propagative manner. In this study, we first developed a minireplicon system of NCMV in Nicotiana benthamiana plants, and then recovered a recombinant NCMV virus (rNCMV‐RFP), with a red fluorescent protein (RFP) insertion, in SBPHs and barley plants. We further used rNCMV‐RFP and green fluorescent protein (GFP)‐tagged barley yellow striate mosaic virus (rBYSMV‐GFP), a closely related cytorhabdovirus, to study superinfection exclusion, a widely observed phenomenon in dicot plants rarely studied in monocot plants. Interestingly, cellular superinfection exclusion of rBYSMV‐GFP and rNCMV‐RFP was observed in barley leaves. Our results demonstrate that two insect‐transmitted cytorhabdoviruses are enemies rather than friends at the cellular level during coinfections in plants. The recombinant NCMV‐RFP virus was rescued in barley plants and insect vectors to reveal cellular superinfection exclusion of two cytorhabdoviruses, NCMV‐RFP and BYSMV‐GFP, in barley.
ISSN:1464-6722
1364-3703
DOI:10.1111/mpp.13188