Band 3 Protein: An Effective Interrogation Tool of Storage Lesions in RBC Units

The present study aims to investigate the changes in different parameters related to the storage time of red blood cell (RBC) units. Microscopic, flow cytometric, and electrophoretic assessments were employed every few days for 60 days to investigate the alterations in morphology, size, phosphatidyl...

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Published in:Indian journal of hematology & blood transfusion 2022-04, Vol.38 (2), p.373-380
Main Authors: Ameri, Zahra, Farsinejad, Alireza, Vahidi, Reza, Sheikh Rezaei, Zahra, Khaleghi, Morteza, Parvizi, Poorya, Moghadari, Masoud
Format: Article
Language:English
Subjects:
Blood Transfusion Medicine
Erythrocytes
Hematology
Human Genetics
Medicine
Medicine & Public Health
Microscopy
Morphology
Oncology
Original
Original Article
Proteins
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Summary:The present study aims to investigate the changes in different parameters related to the storage time of red blood cell (RBC) units. Microscopic, flow cytometric, and electrophoretic assessments were employed every few days for 60 days to investigate the alterations in morphology, size, phosphatidylserine (PS) externalization, and membrane proteins over time. Morphological transformation from discocytes to spherocytes progressed as the storage time increased, which was accompanied by an increment of cellular size. However, this storage period did not result in the externalization of significant amounts of PS ( p  > 0.05). Mean Fluorescence Intensity (MFI) values increased by 11% to 23% between days 21 and 35 compared to the day 1 sample ( p  
ISSN:0971-4502
0974-0449
0974-0449
0971-4502
DOI:10.1007/s12288-021-01447-4