Band 3 Protein: An Effective Interrogation Tool of Storage Lesions in RBC Units

The present study aims to investigate the changes in different parameters related to the storage time of red blood cell (RBC) units. Microscopic, flow cytometric, and electrophoretic assessments were employed every few days for 60 days to investigate the alterations in morphology, size, phosphatidyl...

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Published in:Indian journal of hematology & blood transfusion 2022-04, Vol.38 (2), p.373-380
Main Authors: Ameri, Zahra, Farsinejad, Alireza, Vahidi, Reza, Sheikh Rezaei, Zahra, Khaleghi, Morteza, Parvizi, Poorya, Moghadari, Masoud
Format: Article
Language:English
Subjects:
Blood Transfusion Medicine
Erythrocytes
Hematology
Human Genetics
Medicine
Medicine & Public Health
Microscopy
Morphology
Oncology
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Proteins
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container_title Indian journal of hematology & blood transfusion
container_volume 38
creator Ameri, Zahra
Farsinejad, Alireza
Vahidi, Reza
Sheikh Rezaei, Zahra
Khaleghi, Morteza
Parvizi, Poorya
Moghadari, Masoud
description The present study aims to investigate the changes in different parameters related to the storage time of red blood cell (RBC) units. Microscopic, flow cytometric, and electrophoretic assessments were employed every few days for 60 days to investigate the alterations in morphology, size, phosphatidylserine (PS) externalization, and membrane proteins over time. Morphological transformation from discocytes to spherocytes progressed as the storage time increased, which was accompanied by an increment of cellular size. However, this storage period did not result in the externalization of significant amounts of PS ( p  > 0.05). Mean Fluorescence Intensity (MFI) values increased by 11% to 23% between days 21 and 35 compared to the day 1 sample ( p  
doi_str_mv 10.1007/s12288-021-01447-4
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Microscopic, flow cytometric, and electrophoretic assessments were employed every few days for 60 days to investigate the alterations in morphology, size, phosphatidylserine (PS) externalization, and membrane proteins over time. Morphological transformation from discocytes to spherocytes progressed as the storage time increased, which was accompanied by an increment of cellular size. However, this storage period did not result in the externalization of significant amounts of PS ( p  &gt; 0.05). Mean Fluorescence Intensity (MFI) values increased by 11% to 23% between days 21 and 35 compared to the day 1 sample ( p  &lt; 0.001). By day 60, the MFI decreased to about 70% of the day 1 sample. The analysis of membrane proteins' distribution showed a significant drop in band 3 expression after 35 days ( p  &lt; 0.05 and 0.001 on days 42 and 60, respectively); however, no significant change was observed up to five weeks ( p  &gt; 0.05). 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The inconsistency observed between Eosin-5-Maleimide (5-EMA) binding and the relative band 3 content could be due to additional accessibility of 5-EMA to hidden domains of other membrane proteins on RBCs as a result of increased mean corpuscular volume (MCV) and changes in morphology. 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subjects Blood Transfusion Medicine
Erythrocytes
Hematology
Human Genetics
Medicine
Medicine & Public Health
Microscopy
Morphology
Oncology
Original
Original Article
Proteins
title Band 3 Protein: An Effective Interrogation Tool of Storage Lesions in RBC Units
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