Insights Into Pneumococcal Pneumonia Using Lung Aspirates and Nasopharyngeal Swabs Collected From Pneumonia Patients in The Gambia

Abstract Background We investigated the pathogenesis of pneumococcal pneumonia using clinical specimens collected for pneumonia surveillance in The Gambia. Methods Lung aspirates and nasopharyngeal swabs from 31 patients were examined by culture, quantitative polymerase chain reaction (qPCR), whole...

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Bibliographic Details
Published in:The Journal of infectious diseases 2022-04, Vol.225 (8), p.1447-1451
Main Authors: Dunne, Eileen M, Hua, Yinglei, Salaudeen, Rasheed, Hossain, Ilias, Ndiaye, Malick, Ortika, Belinda D, Mulholland, E Kim, Hinds, Jason, Manna, Sam, Mackenzie, Grant A, Satzke, Catherine
Format: Article
Language:English
Subjects:
Child
Gambia - epidemiology
Gene expression
Genomes
Humans
Lung
Lungs
Major and Brief Reports
Nasopharynx
Pathogenesis
Patients
Pediatrics
Pneumococcal Infections - diagnosis
Pneumolysin
Pneumonia
Pneumonia, Pneumococcal - diagnosis
Serotyping
Streptococcus infections
Streptococcus pneumoniae - genetics
Virulence
Whole genome sequencing
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Summary:Abstract Background We investigated the pathogenesis of pneumococcal pneumonia using clinical specimens collected for pneumonia surveillance in The Gambia. Methods Lung aspirates and nasopharyngeal swabs from 31 patients were examined by culture, quantitative polymerase chain reaction (qPCR), whole genome sequencing, serotyping, and reverse-transcription qPCR. Results Five lung aspirates cultured pneumococci, with a matching strain identified in the nasopharynx. Three virulence genes including ply (pneumolysin) were upregulated >20-fold in the lung compared with the nasopharynx. Nasopharyngeal pneumococcal density was higher in pediatric pneumonia patients compared with controls (P 
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/jiaa186