Evaluation and optimization of PolyJet 3D-printed materials for cell culture studies

• Published in: Analytical and bioanalytical chemistry 2022-05, Vol.414 (11), p.3329-3339
• Main Authors: Currens, Emily R., Armbruster, Michael R., Castiaux, Andre D., Edwards, James L., Martin, R. Scott
• Format: Article
• Language: English
• Subjects: 3-D printers; 3D printing; Analysis; Analytical Chemistry; Animals; Biochemistry; Cattle; Cell culture; Cell Culture Techniques; Cell density; Cell lines; Cell morphology; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Chromatography, Liquid; Culture; Cytology; Devices; Dogs; Endothelial Cells; Flasks; Food Science; Laboratory Medicine; Leachates; Methods; Microfluidics; Monitoring/Environmental Analysis; Optimization; Paper in Forefront; Printed materials; Printing; Printing, Three-Dimensional; Pulmonary arteries; Pulmonary artery; Sodium hydroxide; Sodium silicates; Substrates; Tandem Mass Spectrometry; Three dimensional printing
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-022-03991-y

Use of 3D printing for microfluidics is a rapidly growing area, with applications involving cell culture in these devices also becoming of interest. 3D printing can be used to create custom-designed devices that have complex features and integrate different material types in one device; however, there are fewer studies studying the ability to culture cells on the various substrates that are available. This work describes the effect of PolyJet 3D-printing technology on cell culture of two cell lines, bovine pulmonary artery endothelial cells (BPAECs) and Madin-Darby Canine Kidney (MDCK) cells, on two different types of printed materials (VeroClear or MED610). It was found that untreated devices, when used for studies of 1 day or more, led to unsuccessful culture. A variety of device treatment methodologies were investigated, with the most success coming from the use of sodium hydroxide/sodium metasilicate solution. Devices treated with this cleaning step resulted in culture of BPAECs and MDCK cells that were more similar to what is obtained in traditional culture flasks (in terms of cell morphology, viability, and cell density). LC–MS/MS analysis (via Orbitrap MS) was used to determine potential leachates from untreated devices. Finally, the use of a fiber scaffold in the devices was utilized to further evaluate the treatment methodology and to also demonstrate the ability to perform 3D culture in such devices. This study will be of use for researchers wanting to utilize these or other cell types in PolyJet-based 3D-printed devices.