Performance of the QIAprep&amp Viral RNA UM Kit assay (Qiagen), an automatable method for RT-qPCR detection of SARS-CoV-2 without RNA extraction

•Accurate identification of patients at risk of transmission.•Direct RT-qPCR on heat inactivated and lysed samples.•It is a rapid and automatable method, no extraction required. We evaluated the performance of the QIAprep&amp Viral RNA UM Kit (Qiagen) for SARS-CoV-2 detection. It displayed speci...

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Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2022-07, Vol.103 (3), p.115700-115700, Article 115700
Main Authors: Fenaux, Honorine, Limam, Lamia, Soutiere, Marie-Pierre, Veillet, Frédéric, Escuret, Vanessa, Roque-Afonso, Anne-Marie
Format: Article
Language:English
Subjects:
cellular control
low volumes
rapid diagnosis
RT-qPCR
SARS-CoV-2
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Summary:•Accurate identification of patients at risk of transmission.•Direct RT-qPCR on heat inactivated and lysed samples.•It is a rapid and automatable method, no extraction required. We evaluated the performance of the QIAprep&amp Viral RNA UM Kit (Qiagen) for SARS-CoV-2 detection. It displayed specificity and sensitivity required for SARS-CoV-2 RNA detection from swab transport media without RNA extraction. This method identifies accurately patients at risk of transmission while saving time and cost of extraction.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2022.115700