PcWRKY11, an II-d WRKY Transcription Factor from Polygonum cuspidatum , Enhances Salt Tolerance in Transgenic Arabidopsis thaliana

• Published in: International journal of molecular sciences 2022-04, Vol.23 (8), p.4357
• Main Authors: Wang, Guowei, Wang, Xiaowei, Ma, Hongping, Fan, Haili, Lin, Fan, Chen, Jianhui, Chai, Tuanyao, Wang, Hong
• Format: Article
• Language: English
• Subjects: Abiotic stress; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis thaliana; Ascorbic acid; Calcium-binding protein; Calmodulin; Catalase; Domains; Enzymatic activity; Enzyme activity; Fallopia japonica - genetics; Fallopia japonica - metabolism; Gene expression; Gene Expression Regulation, Plant; Homology; L-Ascorbate peroxidase; Localization; Metabolism; Methyl jasmonate; Molecular modelling; Nucleotide sequence; Osmosis; Osmotic stress; Peroxidase; Phylogenetics; Plant Proteins - genetics; Plant Proteins - metabolism; Plants, Genetically Modified - genetics; Plants, Genetically Modified - metabolism; Polygonum cuspidatum; Proteins; Reactive oxygen species; Salinity; Salinity tolerance; Salt Tolerance - genetics; Signal transduction; Sodium chloride; Stress, Physiological - genetics; Superoxide dismutase; Superoxide Dismutase - metabolism; Transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism; Transcriptomes; Transgenic plants
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms23084357

Being an invasive plant, is highly resilient and can survive in unfavorable environments for long periods; however, its molecular mechanisms associated with such environmental resistance are largely unknown. In this study, a WRKY transcription factor (TF) gene, , was identified from by analyzing methyl jasmonate (MeJA)-treated transcriptome data. It showed a high degree of homology with WRKY11 from , containing a WRKY domain and a zinc finger structure and II-d WRKY characteristic domains of HARF, a calmodulin-binding domain (C-motif), and a putative nuclear localization signal (NLS) through sequence alignment and functional element mining. qPCR analysis showed that the expression of PcWRKY11 can be induced by NaCl, osmotic stress, and UV-C. In this study, we also found that overexpression of in could significantly increase salt tolerance. To explore its possible molecular mechanism, further investigations showed that compared with the wild type (WT), under salt stress, the transgenic plants showed a lower malondialdehyde (MDA) content, higher expression of ) and ( ), and higher enzyme activity of peroxidase POD), superoxide dismutase (SOD), and catalase (CAT). Moreover, the transgenic plants also showed higher expression of Δ -pyrroline-5-carboxylate synthase ( ), and higher contents of proline and soluble sugar. Taken together, these results indicate that may have a positive role in plants' adaptation to salinity conditions by reducing reactive oxygen species (ROS) levels and increasing osmosis substance synthesis.