From Angstroms to Nanometers: Measuring Interatomic Distances by Solid-State NMR

Internuclear distances represent one of the main structural constraints in molecular structure determination using solid-state NMR spectroscopy, complementing chemical shifts and orientational restraints. Although a large number of magic-angle-spinning (MAS) NMR techniques have been available for di...

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Bibliographic Details
Published in:Chemical reviews 2022-05, Vol.122 (10), p.9848-9879
Main Authors: Shcherbakov, Alexander A., Medeiros-Silva, João, Tran, Nhi, Gelenter, Martin D., Hong, Mei
Format: Article
Language:English
Subjects:
Distance measurement
Experiments
Gyromagnetic ratio
Macromolecules
Magnetic Resonance Imaging
Magnetic Resonance Spectroscopy
Molecular structure
NMR spectroscopy
Proteins - chemistry
Solid state
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Summary:Internuclear distances represent one of the main structural constraints in molecular structure determination using solid-state NMR spectroscopy, complementing chemical shifts and orientational restraints. Although a large number of magic-angle-spinning (MAS) NMR techniques have been available for distance measurements, traditional 13C and 15N NMR experiments are inherently limited to distances of a few angstroms due to the low gyromagnetic ratios of these nuclei. Recent development of fast MAS triple-resonance 19F and 1H NMR probes has stimulated the design of MAS NMR experiments that measure distances in the 1–2 nm range with high sensitivity. This review describes the principles and applications of these multiplexed multidimensional correlation distance NMR experiments, with an emphasis on 19F- and 1H-based distance experiments. Representative applications of these long-distance NMR methods to biological macromolecules as well as small molecules are reviewed.
ISSN:0009-2665
1520-6890
DOI:10.1021/acs.chemrev.1c00662