Chemical and biological studies on the soft coral Nephthea sp

• Published in: RSC advances 2021-07, Vol.11 (38), p.23654-23663
• Main Authors: Abdelhafez, Omnia Hesham, Fahim, John Refaat, El Masri, Ramy R, Salem, M Alaraby, Desoukey, Samar Yehia, Ahmed, Safwat, Kamel, Mohamed Salah, Pimentel-Elardo, Sheila Marie, Nodwell, Justin R, Abdelmohsen, Usama Ramadan
• Format: Article
• Language: English
• Subjects: Annotations; Antiproliferatives; Butanol; Cancer; Chemistry; Corals; Kinases; Leukemia; Metabolism; Metabolites; Molecular docking; Molecular interactions; Nanoparticles; Proteins; Silver; Terpenes; Tumors; Tyrosine
• ISSN: 2046-2069; 2046-2069
• DOI: 10.1039/d1ra03045k

Soft corals belonging to the family Nephtheidae have been appreciated as marine sources of diverse metabolites with promising anticancer potential. In view of that, the current work investigates the anti-proliferative potential of the crude extract, different fractions, and green synthesized silver nanoparticles (AgNPs) of the Red Sea soft coral, sp. against a panel of tumor cell lines. The metabolic pool of the soft coral under study was also explored an LC-HR-ESI-MS metabolomics approach, followed by molecular docking analysis of the characterized metabolites against the target proteins, EGFR, VEGFR, and HER2 (erbB2) that are known to be involved in cancer cell proliferation, growth, and survival. Overall, the -butanol fraction of sp. exhibited the highest inhibitory activities against MCF7 (breast cancer) and A549 (lung cancer) cell lines, with interesting IC values of 2.30 ± 0.07 and 3.12 ± 0.10 μg ml , respectively, whereas the maximum growth inhibition of HL60 (leukemia) cells was recorded by the total extract (IC = 2.78 ± 0.09 μg ml ). More interestingly, the anti-proliferative potential of the total soft coral extract was evidently improved when packaged in the form of biogenic AgNPs, particularly against A549 and MCF7 tumor cells, showing IC values of 0.72 ± 0.06 and 9.32 ± 0.57 μg ml , respectively. On the other hand, metabolic profiling of sp. resulted in the annotation of structurally diverse terpenoids, some of which displayed considerable binding affinities and molecular interactions with the studied target proteins, suggesting their possible contribution to the anti-proliferative properties of sp. inhibition of tyrosine kinases, especially the EGFR type. Taken together, the present findings highlighted the relevance of sp. to future anticancer drug discovery and provided a base for further work on the green synthesis of a range of bioactive NPs from marine soft corals.