PLGA nanoparticle preparations by emulsification and nanoprecipitation techniques: effects of formulation parameters

This study presents the influence of the primary formulation parameters on the formation of poly-dl-lactic- -glycolic nanoparticles by the emulsification-solvent evaporation, and the nanoprecipitation techniques. In the emulsification-solvent evaporation technique, the polymer and tensoactive concen...

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Bibliographic Details
Published in:RSC advances 2020-01, Vol.10 (8), p.4218-4231
Main Authors: Hernández-Giottonini, Karol Yesenia, Rodríguez-Córdova, Rosalva Josefina, Gutiérrez-Valenzuela, Cindy Alejandra, Peñuñuri-Miranda, Omar, Zavala-Rivera, Paul, Guerrero-Germán, Patricia, Lucero-Acuña, Armando
Format: Article
Language:English
Subjects:
Chemistry
Cryoprotectants
Electrophoresis
Emulsification
Evaporation
Ions
Nanoparticles
Photon correlation spectroscopy
Polymers
Process parameters
Solvents
Statistical analysis
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Summary:This study presents the influence of the primary formulation parameters on the formation of poly-dl-lactic- -glycolic nanoparticles by the emulsification-solvent evaporation, and the nanoprecipitation techniques. In the emulsification-solvent evaporation technique, the polymer and tensoactive concentrations, the organic solvent fraction, and the sonication amplitude effects were analyzed. Similarly, in the nanoprecipitation technique the polymer and tensoactive concentrations, the organic solvent fraction and the injection speed were varied. Additionally, the agitation speed during solvent evaporation, the centrifugation speeds and the use of cryoprotectants in the freeze-drying process were analyzed. Nanoparticles were characterized by dynamic light scattering, laser Doppler electrophoresis, and scanning electron microscopy, and the results were evaluated by statistical analysis. Nanoparticle physicochemical characteristics can be adjusted by varying the formulation parameters to obtain specific sizes and stable nanoparticles. Also, by adjusting these parameters, the nanoparticle preparation processes have the potential to be tuned to yield nanoparticles with specific characteristics while maintaining reproducible results.
ISSN:2046-2069
2046-2069
DOI:10.1039/c9ra10857b