Mycobacterium tuberculosis encodes a YhhN family membrane protein with lysoplasmalogenase activity that protects against toxic host lysolipids

• Published in: The Journal of biological chemistry 2022-05, Vol.298 (5), p.101849, Article 101849
• Main Authors: Jurkowitz, Marianne S., Azad, Abul K., Monsma, Paula C., Keiser, Tracy L., Kanyo, Jean, Lam, TuKiet T., Bell, Charles E., Schlesinger, Larry S.
• Format: Article
• Language: English
• Subjects: cell injury and protection; cell wall–deficient; Humans; Hydrolases - genetics; Hydrolases - metabolism; Lysophosphatidylcholines; Lysophospholipids; lysoplasmalogen/lysophospholipid; lysoplasmalogenase; macrophage; Macrophages - microbiology; Membrane Proteins - genetics; Membrane Proteins - metabolism; Mycobacterium smegmatis; Mycobacterium tuberculosis - enzymology; Mycobacterium tuberculosis - genetics; Mycobacterium tuberculosis Rv1401 gene; phospholipase A; Plasmalogens - metabolism; spheroplasts; YhhN protein
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/j.jbc.2022.101849

The pathogen Mycobacterium tuberculosis (M.tb) resides in human macrophages, wherein it exploits host lipids for survival. However, little is known about the interaction between M.tb and macrophage plasmalogens, a subclass of glycerophospholipids with a vinyl ether bond at the sn-1 position of the glycerol backbone. Lysoplasmalogens, produced from plasmalogens by hydrolysis at the sn-2 carbon by phospholipase A2, are potentially toxic but can be broken down by host lysoplasmalogenase, an integral membrane protein of the YhhN family that hydrolyzes the vinyl ether bond to release a fatty aldehyde and glycerophospho-ethanolamine or glycerophospho-choline. Curiously, M.tb encodes its own YhhN protein (MtbYhhN), despite having no endogenous plasmalogens. To understand the purpose of this protein, the gene for MtbYhhN (Rv1401) was cloned and expressed in Mycobacterium smegmatis (M.smeg). We found the partially purified protein exhibited abundant lysoplasmalogenase activity specific for lysoplasmenylethanolamine or lysoplasmenylcholine (pLPC) (Vmax∼15.5 μmol/min/mg; Km∼83 μM). Based on cell density, we determined that lysoplasmenylethanolamine, pLPC, lysophosphatidylcholine, and lysophosphatidylethanolamine were not toxic to M.smeg cells, but pLPC and LPC were highly toxic to M.smeg spheroplasts, which are cell wall–deficient mycobacterial forms. Importantly, spheroplasts prepared from M.smeg cells overexpressing MtbYhhN were protected from membrane disruption/lysis by pLPC, which was rapidly depleted from the media. Finally, we found that overexpression of full-length MtbYhhN in M.smeg increased its survival within human macrophages by 2.6-fold compared to vector controls. These data support the hypothesis that MtbYhhN protein confers a growth advantage for mycobacteria in macrophages by cleaving toxic host pLPC into potentially energy-producing products.